Effects of 4000-rad irradiation on the in vitro storage properties of packed red cells. Final report
Immunosuppressed patients who require red cell transfusions receive irradiated (1500-3000 rad) packed red cells. These cells are irradiated immediately before infusion. If a large group of patients become immunosuppressed due to exposure to radiation or chemicals, the ability to supply large volumes of irradiated blood at the time of use might not be possible. An alternate solution to providing quantities of irradiated blood is to irradiate the units prior to storage. This study presents in vitro data comparing storage of paired packed red cell units either irradiated or not irradiated. Five units of fresh blood drawn into citrate-phosphate-dextrose-adenine (CPDA-1) were packed to a hematocrit of 75 + or - 1%, and then each unit was divided in two equal parts. One of each pair was irradiated (4000 rads), and both parts of each unit were stored for 35 days at 4 C. Samples were analyzed every 7 days. Irradiation caused a slight drop in red cell adenosine triphosphate and 2,3 diphosphoglycerate and a slight increase in plasma hemoglobin compared to controls. Methemoglobin, pH, and glucose consumption were identical to the controls. The evidence indicates that irradiation did not cause biochemical or metabolic changes in the red cells that would lead us to suspect a difference between irradiated and nonirradiated stored red cells in function or viability. These negative findings require in vivo confirmation.
- Research Organization:
- Letterman Army Inst. of Research, San Francisco, CA (USA)
- OSTI ID:
- 5686853
- Report Number(s):
- AD-A-164873/2/XAB
- Country of Publication:
- United States
- Language:
- English
Similar Records
Effects of irradiation on red cells stored in CPDA-1 and CPD-ADSOL (AS-1)
Effects of storage on irradiated red blood cells: An in-vitro and in-vivo study. Master's thesis
Related Subjects
BLOOD PLASMA
IRRADIATION
ERYTHROCYTES
BIOLOGICAL RADIATION EFFECTS
PATIENTS
IMMUNOSUPPRESSION
BLOOD
BLOOD CHEMISTRY
GLUCOSE
HEMOGLOBIN
METHEMOGLOBIN
STORAGE
ALDEHYDES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CARBOXYLIC ACIDS
GLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HEXOSES
MATERIALS
MONOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PIGMENTS
PORPHYRINS
PROTEINS
RADIATION EFFECTS
SACCHARIDES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture