Intracellular and extracellular radiosensitization of Serratia marcescens by bipyridinium compounds. [Benzylviologen and methylviologen]
The one-electron reduced form of the bipyridinium compounds benzylviologen and methylviologen have been found to diffuse across the cytoplasmic membrane of Serratia marcescens cells. Subsequent reoxidation of the viologens to the dicationic form traps the compound inside the cells. Cells at a density of 4 x 10/sup 9/ ml/sup -1/ took up approximately half of the compound when incubated with an initial extracellular concentration of 200..mu..M of either reduced viologen. The degree of radiosensitization in anoxia afforded by the compounds parallels the rise in internal concentration and reaches a maximum enhancement ratio of 2.0 +- 0.1 for both compounds. This level in sensitization is similar to that found when the compounds are external to the cell. No additivity in sensitization is found when the viologens are both internal and external to the cells at the time of irradiation suggesting that the same target site is sensitized. This site is probably some membrane-associated structure.
- Research Organization:
- Mount Vernon Hospital, Northwood, England
- OSTI ID:
- 5686126
- Journal Information:
- Radiat. Res.; (United States), Vol. 19:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
PYRIDINIUM COMPOUNDS
MEMBRANE TRANSPORT
RADIOSENSITIVITY EFFECTS
SERRATIA
BIOLOGICAL RADIATION EFFECTS
ANOXIA
COBALT 60
EXPERIMENTAL DATA
GAMMA RADIATION
IRRADIATION
ISOLATED VALUES
REDOX REACTIONS
SOLUTIONS
SURVIVAL CURVES
AMINES
AMMONIUM COMPOUNDS
AZINES
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
CHEMICAL REACTIONS
COBALT ISOTOPES
DATA
DATA FORMS
DISPERSIONS
ELECTROMAGNETIC RADIATION
HETEROCYCLIC COMPOUNDS
INFORMATION
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MICROORGANISMS
MINUTES LIVING RADIOISOTOPES
MIXTURES
NUCLEI
NUMERICAL DATA
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIDINES
QUATERNARY COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
YEARS LIVING RADIOISOTOPES
560121* - Radiation Effects on Cells- External Source- (-1987)
550200 - Biochemistry