Radioimmunoassay of gut regulatory peptides

Book · Fri Jan 01 04:00:00 EST 1982

OSTI ID:5685494

Bloom, R; Long, R G ^[1]

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1. eds.

This multiauthored book describes the methodology used to quantiate several gut regulatory peptides. The test assumes some basic radioimmunoassay knowledge, as there is little general theory presented. The first several chapters discuss, in varying detail, general factors of RIA including reagent preparation, tissue extraction, and the problems associated with measurements in extracts and plasma. The authors present good, step-by-step methods for antibody production and peptide iodination. Chapters 8 to 20 each give the general chemistry, localization, physiology, pharmacology, biological concentration, and pathological considerations for each of the most commonly recognized gut regulatory peptides, such as gastrin, cholecystokinin, secretin, vasoactive intestinal polypeptide, gastric inhibitory polypeptide, glucagon, and others, and the neuropeptides such as bombesin and substance P. The chapters also include reagent preparation and assay parameters.

• OSTI ID: 5685494
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
DIGESTIVE SYSTEM
GASTRIN
GASTROINTESTINAL TRACT
GLUCAGON
HORMONES
ISOTOPE APPLICATIONS
KININS
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PEPTIDES
PHARMACOLOGY
PHYSIOLOGY
POLYPEPTIDES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
REAGENTS
SECRETIN
TRACER TECHNIQUES