Convenient radioimmunoassay for urinary human choriogonadotropin without interference by urinary human lutropin

Journal Article · Mon Nov 30 23:00:00 EST 1981 · Clin. Chem. (Winston-Salem, N.C.); (United States)

OSTI ID:5682239

Wehmann, R E ^[1]; Harman, S M; Birken, S; Canfield, R E; Nisula, B C

---

1. Baltimore City Hospitals, MD

We have devised a radioimmunoassay (RIA) for human choriogonadotropin (hCG) in first morning-voided urine specimens. Concanavalin A, a lectin, is used to extract and concentrate the hCG from urine. A high-affinity antiserum is used, directed to the hCG..beta.. carboxy-terminal peptide, a unique immunological determinant not shared by the beta subunit of human lutropin. This ensures that urinary human lutropin-related molecules, which interfere with RIAs involving antisera to the intact hCG..beta.. subunit, will not cross react in this assay. A concentration of hCG as low as 0.4 ..mu..g/L can be detected in the first morning-voided urine. The effective sensitivity of this assay for the unequivocal detection of hCG production is somewhat better than that achieved with the serum hCG RIA involving antisera to the hCG..beta.. subunit. The improved specificity and sensitivity of this assay, and the greater convenience of collecting samples of urine rather than blood, are clinically useful advantages of this approach to assessing hCG production in humans.

• OSTI ID: 5682239
• Journal Information: Clin. Chem. (Winston-Salem, N.C.); (United States), Journal Name: Clin. Chem. (Winston-Salem, N.C.); (United States) Vol. 27:12; ISSN CLCHA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
AGGLUTININS
ANTIBODIES
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
CONCANAVALIN
DATA
DIAGNOSIS
EXPERIMENTAL DATA
GONADOTROPINS
HCG
HEMAGGLUTININS
HORMONES
IMMUNE SERUMS
INFORMATION
INHIBITION
ISOTOPE APPLICATIONS
LH
MATERIALS
NUMERICAL DATA
PATIENTS
PEPTIDE HORMONES
PITUITARY HORMONES
RADIOASSAY
RADIOIMMUNOASSAY
TRACER TECHNIQUES
URINE
WASTES