Construction and availability of human chromosome-specific gene libraries

Conference · Fri Jun 14 04:00:00 EDT 1985

OSTI ID:5682133

Fuscoe, J C; Van Dilla, M A; Deaven, L L

This report briefly describes Phase I of the project, the production of complete digest fibraries. Each laboratory is currently in the process of sorting individual human chromosomes from normal human fibroblasts or human X hamster hybrids. The goal of 4 x 10/sup 6/ chromosomes for cloning purposes has been achieved. Each laboratory is also in the process of cloning the chromosomal DNA, after complete digestion with a 6-cutter, into the bacteriophage vector Charon 21A. 3 refs.

• Research Organization: Lawrence Livermore National Lab., CA (USA); Los Alamos National Lab., NM (USA)
• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 5682133
• Report Number(s): UCRL-92837; CONF-8506137-2; ON: DE85013981
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BACTERIOPHAGES
CELL FLOW SYSTEMS
CHROMOSOMES
CLONING
CONNECTIVE TISSUE CELLS
DNA
DNA-CLONING
FIBROBLASTS
LIBRARIES
MAMMALS
MAN
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PRIMATES
SOMATIC CELLS
SORTING
VERTEBRATES
VIRUSES