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Characterization of voltage calcium channels in a clonal pituitary cell line

Journal Article · · Life Sci.; (United States)
The authors have pharmacologically characterized voltage sensitive calcium channels (VSCCs) in GH/sub 3/ cells, an anterior pituitary clonal cell line known to secrete prolactin and growth hormone. Raising the medium K/sup +/ concentration from 5 to 50 mM caused an immediate increase in net /sup 45/Ca/sup 2 +/ uptake which remained apparent over a 15 minute time course. /sup 45/Ca/sup 2 +/ uptake was maximally stimulated nearly 10-fold over basal levels. This K/sup +/-induced stimulation of Ca/sup 2 +/ uptake was not prevented by 10-/sup 5/M tetrodotoxin or by replacing sodium with choline in the assay medium. Ca/sup 2 +/ uptake was, however, inhibited by several VSCC antagonists: nitrendipine, D-600, diltiazem and Ca/sup 2 +/. Further, the novel dihydropyridine VSCC agonists, BAY K8644 and CGP 28392, enhanced 50-mM K/sup +/-stimulated /sup 45/Ca/sup 2 +/ uptake and these effects were blocked by nitrendipine. 40 references, 3 figures.
Research Organization:
Univ. of Chicago Pritzker School of Medicine, IL
OSTI ID:
5681978
Journal Information:
Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 36:23; ISSN LIFSA
Country of Publication:
United States
Language:
English