Clearance and binding of radiolabeled glycoproteins by cells of the murine mononuclear phagocyte system
The clearance and binding of radiolabeled lactoferrin and fast ..cap alpha../sub 2/-macroglobulin were studied. Both glycoproteins cleared rapidly following intravenous injection in mice, and both bound specifically to discrete receptors on murine peritoneal macrophages. The simultaneous presence of excess, unlabeled ligands specific for receptors recognizing terminal fucose, mannose, N-acetylglucosamine or galactose residues did not inhibit the clearance or binding of either lactoferrin or fast-..cap alpha../sub 2/M. The clearance and binding of enzymatically defucosylated lactoferrin was indistinguishable from native lactoferrin, indicating that terminal ..cap alpha..(1-3)-linked fucose on lactoferrin is not necessary for receptor recognition. The clearance and binding of two fast -..cap alpha../sub 2/M forms, ..cap alpha../sub 2/M-trypsin and ..cap alpha../sub 2/M-MeNH/sub 2/ cross compete with each other. Saturation binding studies indicated that the total binding of mannosyl -BSA, fusocyl-BSA, and N-acetylglucosaminyl-BSA to macrophages activated by BCG was approximately 15% of the levels observed with inflammatory macrophages elicited by thioglycollate broth. Cross-competition binding studies demonstrated a common surface receptor mediated binding of all three neoglycoprotein ligands and was identical to the receptor on mononuclear phagocytes that binds mannosyl- and N-acetylglucosaminyl-terminated glycoproteins. These results suggest that difference between discrete states of macrophage function may be correlated with selective changes in levels of the surface receptor for mannose-containing glycoproteins.
- Research Organization:
- Duke Univ., Durham, NC (USA)
- OSTI ID:
- 5681671
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
AMINES
ANIMAL CELLS
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL PATHWAYS
CARBOHYDRATES
CHEMICAL BONDS
CLEARANCE
DATA
EXPERIMENTAL DATA
GALACTOSE
GLOBULINS
GLOBULINS-ALPHA
GLUCOPROTEINS
GLUCOSAMINE
HEXOSAMINES
HEXOSES
INACTIVATION
INFORMATION
INJECTION
INTAKE
INTRAVENOUS INJECTION
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LACTOFERRIN
LIGANDS
MAMMALS
MANNOSE
MEMBRANE PROTEINS
MEMBRANES
METALLOPROTEINS
MICE
MONOSACCHARIDES
NUMERICAL DATA
ORGANIC COMPOUNDS
PERITONEUM
PHAGOCYTES
PROTEINS
RADIORECEPTOR ASSAY
REACTION KINETICS
RECEPTORS
RODENTS
SACCHARIDES
SATURATION
SEROUS MEMBRANES
SOMATIC CELLS
TRACER TECHNIQUES
VERTEBRATES