Changes of ribulose bisphosphate carboxylase/oxygenase content, ribulose bisphosphate concentration, and photosynthetic activity during adaptation of high-CO/sub 2/ grown cells to low-CO/sub 2/ conditions in Chlorella pyrenoidosa

Journal Article · Sat Feb 01 04:00:00 EST 1986 · Plant Physiol.; (United States)

OSTI ID:5681570

Yokota, A; Canvin, D T

Changes of some photosynthetic properties of high-CO/sub 2/ grown cells of Chlorella pyrenoidosa during adaptation to low-CO/sub 2/ conditions have been investigated. The K/sub m/ value of photosynthesis of the high-CO/sub 2/ grown cells for dissolved inorganic carbon was 3.3 millimolar and decreased to 25 to 30 micromolar within 4 hours after transferring to air. In the presence of saturating CO/sub 2/ concentrations the photosynthetic activity of the high-CO/sub 2/ grown cells was 1.5 times as high as that of the low-CO/sub 2/ grown cells. There was a significant rise of the photosynthetic activity during adaptation of the high-CO/sub 2/ grown cells to air, followed by a steady decrease. The activity of ribulose 1,5-bisphosphate carboxylase/oxygenase in both the high and low-CO/sub 2/ grown cells was close to the photosynthetic activity of the cells. The concentration of ribulose 1,5-bisphosphate (RuBP) was higher in the low-CO/sub 2/ adapting and low-CO/sub 2/ grown celsl than in the high-CO/sub 2/ grown cells regardless of the photosynthetic rate. This seems to be due to an increased RuBP regeneration activity during adaptation followed by maintenance of the new higher concentration. The RuBP level always exceeded the concentration of ribulose 1,5-bisphosphate carboxylase/oxygenase RuBP binding sites in both the high- and low-CO/sub 2/ grown cells at any dissolved inorganic carbon concentration.

• Research Organization: Queen's Univ., Kingston, Ontario
• OSTI ID: 5681570
• Journal Information: Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 80:2; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

550500* -- Metabolism
560303 -- Chemicals Metabolism & Toxicology-- Plants-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALGAE
BIOLOGICAL ADAPTATION
BIOLOGICAL EFFECTS
CARBOHYDRATES
CARBON
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CARBON-CARBON LYASES
CARBOXY-LYASES
CARBOXYLASE
CHALCOGENIDES
CHEMICAL BONDS
CHEMICAL REACTIONS
CHLORELLA
DATA
ELEMENTS
ENZYME REACTIVATION
ENZYMES
EXPERIMENTAL DATA
INFORMATION
KETONES
LYASES
MICROORGANISMS
MONOSACCHARIDES
NONMETALS
NUMERICAL DATA
ORGANIC COMPOUNDS
OXIDES
OXIDOREDUCTASES
OXYGEN COMPOUNDS
OXYGENASES
PENTOSES
PHOTOCHEMICAL REACTIONS
PHOTOSYNTHESIS
PLANT CELLS
PLANTS
QUANTITY RATIO
RIBULOSE
SACCHARIDES
SATURATION
SYNTHESIS
UNICELLULAR ALGAE