The effect of two topoisomerase inhibitors on low-dose hypersensitivity and increased radioresistance in Chinese hamster V79 cells

Journal Article · Fri Apr 01 04:00:00 EST 1994 · Radiation Research

DOI:https://doi.org/10.2307/3578777· OSTI ID:56782

Skov, K; Zhou, H; Marples, B ^[1]

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1. British Columbia Cancer Research Centre, Vancouver (Canada)

A preliminary investigation of the effect of topoisomerase inhibitors on the structure of the survival curve at low doses has been carried out in Chinese hamster V79 cells, where there is a deviation from the predicted response to radiation. Cells were treated with one representative drug for each enzyme (topoisomerase I or II) prior to X irradiation in air and assessed for cell survival using automated microscopic location of cells. VP-16 causes little or no effect, while camptothecin has a measurable effect up to 2 Gy. The results are discussed in terms of the role of DNA damage and cell cycle in increased radioresistance, which encourage further investigation of the effects of this class of drugs at low doses. 17 refs., 2 figs.

• Sponsoring Organization: USDOE
• OSTI ID: 56782
• Report Number(s): CONF-9305202--
• Journal Information: Radiation Research, Journal Name: Radiation Research Journal Issue: 1 Vol. 138; ISSN 0033-7587; ISSN RAREAE
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
56 BIOLOGY AND MEDICINE
APPLIED STUDIES
CELL CULTURES
CELL CYCLE
DNA
DOSE-RESPONSE RELATIONSHIPS
ENZYME INHIBITORS
ISOMERASES
RADIOSENSITIVITY EFFECTS
RADIOSENSITIZERS
SURVIVAL CURVES
X RADIATION