Dose-rate effect for DNA damage induced by ionizing radiation in human tumor cells

Journal Article · Fri Apr 01 04:00:00 EST 1994 · Radiation Research

DOI:https://doi.org/10.2307/3578771· OSTI ID:56776

Ruiz de Almodovar, J M ^[1]; Bush, C; Peacock, J H; Steel, G G; Whitaker, S J; McMillan, T J ^[2]

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1. Universidad de Granada, Espana (Spain)
2. Institute of Cancer Research, Surrey (United Kingdom)

The effect of dose rate on clonogenic cell survival and DNA double-strand breaks (DSBs) has been examined in a human bladder carcinoma cell line, RT112, treated with ionizing radiation. Cell survival changed markedly over the range of dose rates used (0.01-1.28 Gy/min) with the curves becoming shallower and straighter as the dose rate was lowered. Similarly, the number of DSBs measured by pulsed-field gel electrophoresis (PFGE) immediately after irradiation varied with dose rate. Fewer DSBs were detectable after low-dose-rate irradiation. However, when a 4-h repair period was allowed after irradiation, cells treated at all dose rates exhibited approximately the same amount of damage. The final level of unrejoined DSBs, as detected by PFGE, therefore does not correlate with cell survival at different dose rates. 16 refs., 2 figs.

• Sponsoring Organization: USDOE
• OSTI ID: 56776
• Report Number(s): CONF-9305202--
• Journal Information: Radiation Research, Journal Name: Radiation Research Journal Issue: 1 Vol. 138; ISSN 0033-7587; ISSN RAREAE
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
56 BIOLOGY AND MEDICINE
APPLIED STUDIES
BIOLOGICAL RADIATION EFFECTS
BLADDER
CLONE CELLS
DAMAGE
DNA
DNA REPAIR
DOSE RATES
DOSE-RESPONSE RELATIONSHIPS
ELECTROPHORESIS
IONIZING RADIATIONS
STRAND BREAKS
SURVIVAL CURVES
TUMOR CELLS