Clearance of a monoclonal anti-DNA antibody following administration of DNA in normal and autoimmune mice

Journal Article · Tue Apr 01 00:00:00 EST 1986 · Clin. Immunol. Immunopathol.; (United States)

DOI:https://doi.org/10.1016/0090-1229(86)90204-7· OSTI ID:5677203

Jones, F S; Pisetsky, D S; Kurlander, R J

To study the assembly of DNA-anti-DNA complexes in vivo, we have measured the clearance from blood and organ localization of a murine IgG2a monoclonal anti-DNA antibody, called 6/0, following the infusion of DNA intravenously or intraperitoneally. Intraperitoneal DNA caused a profound acceleration of 6/0 anti-DNA clearance that was dose dependent and demonstrable after the infusion of as little as 1.9 microgram per gram of body weight of single-stranded DNA. The antibody was cleared primarily in the liver without increased deposition in the kidney. Intraperitoneal infusions of DNA also accelerated the clearance of 6/0 in autoimmune MRL-lpr/lpr mice. In contrast, intravenous DNA given in comparable doses caused only a slight increase in 6/0 antibody clearance; this accelerated clearance was seen only at low antigen doses and only during the first 10 min following DNA infusion. Using double-radiolabeling techniques, 6/0 and Cl.18, an IgG2ak myeloma protein without anti-DNA activity, were found to disappear from blood at a comparable rate in both B6D2 mice and MRL-lpr/lpr mice. These results suggest that the DNA-anti-DNA immune complexes can form in vivo but that this process is profoundly affected by the manner in which DNA enters the circulation. In addition, the results suggest that DNA-dependent clearance is not a major pathway for anti-DNA metabolism in normal or at least one strain of autoimmune mice.

• Research Organization: Duke Univ. Medical Center, Durham, NC
• OSTI ID: 5677203
• Journal Information: Clin. Immunol. Immunopathol.; (United States), Vol. 1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
MONOCLONAL ANTIBODIES
BLOOD-PLASMA CLEARANCE
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL PATHWAYS
DNA
DOUBLE LABELLING
IMMUNE REACTIONS
MICE
TRACER TECHNIQUES
ANIMALS
ANTIBODIES
CLEARANCE
ISOTOPE APPLICATIONS
KINETICS
LABELLING
MAMMALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
REACTION KINETICS
RODENTS
VERTEBRATES
551001* - Physiological Systems- Tracer Techniques