Effect of bone marrow depletion on prostaglandin E-producing suppressor macrophages in mouse spleen
The i.p. injection of Corynebacterium parvum (CP) into CBA/J mice effected increases in macrophage colony-forming cells (M-CFC) when spleen cells were cultured with L cell culture filtrate as a source of colony-stimulating factor. Significant increases in phagocytic macrophages (M phi) with Fc receptors for IgG2a and IgG2b immune complexes were additionally noted among the spleen cells in these mice. These M phi effectively inhibited Con A-induced lymphocyte proliferation, probably reflecting a 10-fold increase above normal controls in prostaglandin E to 47 ng/3 X 10(6) spleen cells/ml. To determine whether the suppressor M phi are immediate derivatives of splenic M-CFC, we tried to induce suppressor M phi by the injection of CP into mice depleted of bone marrow M-CFC by the earlier administration of the bone-seeking isotope, 89Sr. This procedure reduced M-CFC in the bone marrow to less than 1% of normal for more than 30 days. Monocytes in the blood fell to 5% of normal by day 10 and were 30% on day 30. Levels of resident peritoneal M phi showed relatively little change in this period. By contrast, splenic M-CFC increased to 20-fold higher than the cold 88Sr controls. CP-induced suppressor M phi activity, however, was sharply reduced in 89Sr marrow-depleted mice on day 10, despite the striking increase in M-CFC. There was a threefold increase in the number of phagocytic M phi binding IgG2a immune complexes, with no significant increase in IgG2b binding M phi. The kinetics of recovery of suppressor M phi activity showed that on days 20, 30, and 50 after 89Sr injection the activities reached 20%, 30%, and 70% of the cold control, respectively, and correlated with the recovery of significant levels of M-CFC in the bone marrow. Taken together, these observations suggest that splenic M-CFC are not an immediate source of PGE-suppressor M phi in vivo.
- OSTI ID:
- 5675178
- Journal Information:
- J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 6; ISSN JOIMA
- Country of Publication:
- United States
- Language:
- English
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Kinetics
& Toxicology-- Animals
Plants
Microorganisms
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALINE EARTH ISOTOPES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
BONE MARROW
COLONY FORMATION
CONNECTIVE TISSUE CELLS
DISEASES
EVEN-EVEN NUCLEI
HEMATOPOIETIC SYSTEM
HEMIC DISEASES
IMMUNOSUPPRESSION
INHIBITION
INTERMEDIATE MASS NUCLEI
ISOTOPES
LEUKOPENIA
MACROPHAGES
MAMMALS
MICE
NUCLEI
ORGANS
PHAGOCYTES
PROSTAGLANDINS
RADIATION EFFECTS
RADIOISOTOPES
RODENTS
SOMATIC CELLS
SPLEEN
STRONTIUM 90
STRONTIUM ISOTOPES
TISSUES
VERTEBRATES
YEARS LIVING RADIOISOTOPES