Involvement of Rh blood group polypeptides in the maintenance of aminophospholipid asymmetry

Journal Article · Thu Nov 01 04:00:00 EST 1990 · Biochemistry; (USA)

DOI:https://doi.org/10.1021/bi00497a003· OSTI ID:5674538

Schroit, A J; Connor, J ^[1]; Bloy, C; Carton, J-P ^[2]

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1. The Univ. of Texas M. D. Anderson Cancer Center, Houston (USA)
2. INSERM, Paris (France)

The human erythrocyte (RBC) Rh blood group system consists of a complex of distinct integral membrane polypeptides with physical properties common to the aminophospholipid transporter responsible for the transbilayer movement of phosphatidylserine (PS) in RBC. To assess the involvement of Rh polypeptides in PS translocation, the aminophospholipid translocase was labeled with a photoactivatable PS analogue, {sup 125}I-azido-PS, and with an inhibitor of PS transport, {sup 125}I-labeled 2-(2-pyridyldithio)ethylamine. The ability of monoclonal Rh antibodies to immunoprecipitate the labeled transporter was determined. Immunoprecipitated Rh polypeptides were found to be labeled with the aminophospholipid translocase markers, suggesting that Rh proteins are involved in the transbilayer movement of PS.

• OSTI ID: 5674538
• Journal Information: Biochemistry; (USA), Journal Name: Biochemistry; (USA) Vol. 29:45; ISSN 0006-2960; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ASYMMETRY
AZIDO COMPOUNDS
BETA DECAY RADIOISOTOPES
BIOCHEMISTRY
BIOLOGICAL MARKERS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD GROUPS
BODY FLUIDS
CHEMISTRY
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ERYTHROCYTES
ESTERS
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IODINE 125
IODINE ISOTOPES
ISOTOPES
LABELLING
LIPIDS
MAMMALS
MAN
MATERIALS
MEMBRANE PROTEINS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHOLIPIDS
PRECIPITATION
PRIMATES
PROTEINS
RADIOISOTOPES
SEPARATION PROCESSES
VERTEBRATES