Target oriented drugs against leishmania. Annual summary report no. 2, 1 May 1980-30 April 1981

Technical Report · Sat Jan 31 00:00:00 EST 1981

OSTI ID:5673797

Zehavi, U; El-On, J

Excreted Factor (EF) is a carbohydrate-rich material released by different strains of Leishmania during growth. It has antigenic properties similar to those of the intact parasite and plays a role in the infective process. Isolation and purification of EF is necessary for study of its biological function, its use for diagnostic purposes, its use in immunization experiments, the study of its biosynthesis, and the preparation of inhibitors of particular biosynthetic steps. Purification of EF by affinity chromatography was markedly improved by introducing Ricinus lectin (specific for galactose) column. This enabled us to obtain more reliable amino acid and sugar analysis and will be instrumental in more advanced physical, chemical, and immunological studies. We have developed a radioimmunoassay for leishmaniasis utilizing purified EF. The assay can distinguish between Leishmania strains and once further developed, should prove most valuable for the diagnosis of the disease. EF plays a role in the infective process of Leishmania. We have now shown that surface carbohydrate, related to EF, plays a role in the initial attachment of Leishmania promastigots to macrophages - a stage that is a prelude to their engulfment by the macrophages followed by multiplication in their cells.

• Research Organization: Hebrew Univ., Rehovoth (Israel). Faculty of Agriculture
• OSTI ID: 5673797
• Report Number(s): AD-A-100731/9
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
TRYPANOSOMES
RADIOIMMUNOASSAY
TRYPANOSOMIASIS
DIAGNOSIS
ANTIGENS
BIOSYNTHESIS
CARBOHYDRATES
DRUGS
EXCRETION
PROTEINS
PURIFICATION
CLEARANCE
DISEASES
INFECTIOUS DISEASES
ISOTOPE APPLICATIONS
ORGANIC COMPOUNDS
PARASITIC DISEASES
RADIOASSAY
SYNTHESIS
TRACER TECHNIQUES
550601* - Medicine- Unsealed Radionuclides in Diagnostics