Characteristics and autoradiographic localization of 2-( sup 125 I)iodomelatonin binding sites in Djungarian hamster brain

Journal Article · Tue Aug 01 04:00:00 EDT 1989 · Endocrinology; (USA)

DOI:https://doi.org/10.1210/endo-125-2-1011· OSTI ID:5673552

Duncan, M J; Takahashi, J S; Dubocovich, M L ^[1]

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1. Northwestern Univ. Medical School, Chicago, IL (USA)

These studies investigated the characteristics and regional distribution of 2-({sup 125}I)iodomelatonin binding in Djungarian hamster brain. The results showed that 2-({sup 125}I)iodomelatonin labels two types of binding sites, which resemble the ML-1 and ML-2 melatonin subtypes previously described in other tissues. The 2-({sup 125}I)iodomelatonin binding site identified in whole brain membranes has a nanomolar affinity (Kd = 1.48 +/- 0.26 nM) and biochemical and pharmacological characteristics identical to those of the ML-2 site of Syrian hamster whole brain. The 2-({sup 125}I)iodomelatonin site in the hypothalamus has a picomolar affinity (Kd = 43.4 +/- 5.1 pM) and resembles the ML-1 site of chicken retina. The localization of 2-({sup 125}I)iodomelatonin labeling in autoradiographic studies of the Djungarian hamster brain includes the suprachiasmatic nuclei, the median eminence, the reuniens nucleus, and the paraventricular nucleus of the thalamus.

• OSTI ID: 5673552
• Journal Information: Endocrinology; (USA), Journal Name: Endocrinology; (USA) Vol. 125:2; ISSN ENDOA; ISSN 0013-7227
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
AMINES
ANIMALS
AROMATICS
AUTORADIOGRAPHY
AZAARENES
AZOLES
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL LOCALIZATION
BODY
BRAIN
CELL CONSTITUENTS
CELL MEMBRANES
CENTRAL NERVOUS SYSTEM
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
HAMSTERS
HETEROCYCLIC COMPOUNDS
HYPOTHALAMUS
INDOLES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
KINETICS
MAMMALS
MELATONIN
MEMBRANE PROTEINS
MEMBRANES
NERVOUS SYSTEM
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PROTEINS
PYRROLES
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
RODENTS
TRYPTAMINES
VERTEBRATES