Characteristics and autoradiographic localization of 2-( sup 125 I)iodomelatonin binding sites in Djungarian hamster brain

Journal Article · Tue Aug 01 00:00:00 EDT 1989 · Endocrinology; (USA)

DOI:https://doi.org/10.1210/endo-125-2-1011· OSTI ID:5673552

Duncan, M J; Takahashi, J S; Dubocovich, M L ^[1]

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1. Northwestern Univ. Medical School, Chicago, IL (USA)

These studies investigated the characteristics and regional distribution of 2-({sup 125}I)iodomelatonin binding in Djungarian hamster brain. The results showed that 2-({sup 125}I)iodomelatonin labels two types of binding sites, which resemble the ML-1 and ML-2 melatonin subtypes previously described in other tissues. The 2-({sup 125}I)iodomelatonin binding site identified in whole brain membranes has a nanomolar affinity (Kd = 1.48 +/- 0.26 nM) and biochemical and pharmacological characteristics identical to those of the ML-2 site of Syrian hamster whole brain. The 2-({sup 125}I)iodomelatonin site in the hypothalamus has a picomolar affinity (Kd = 43.4 +/- 5.1 pM) and resembles the ML-1 site of chicken retina. The localization of 2-({sup 125}I)iodomelatonin labeling in autoradiographic studies of the Djungarian hamster brain includes the suprachiasmatic nuclei, the median eminence, the reuniens nucleus, and the paraventricular nucleus of the thalamus.

• OSTI ID: 5673552
• Journal Information: Endocrinology; (USA), Vol. 125:2; ISSN 0013-7227
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
MELATONIN
RECEPTORS
BIOLOGICAL LOCALIZATION
AFFINITY
AUTORADIOGRAPHY
BIOCHEMICAL REACTION KINETICS
BRAIN
CELL MEMBRANES
HAMSTERS
HYPOTHALAMUS
IODINE 125
AMINES
ANIMALS
AROMATICS
AZAARENES
AZOLES
BETA DECAY RADIOISOTOPES
BODY
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
HETEROCYCLIC COMPOUNDS
INDOLES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
KINETICS
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
NERVOUS SYSTEM
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PROTEINS
PYRROLES
RADIOISOTOPES
REACTION KINETICS
RODENTS
TRYPTAMINES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques