Homocitrate is a component of the iron-molybdenum cofactor of nitrogenase

Journal Article · Tue Apr 04 04:00:00 EDT 1989 · Biochemistry; (USA)

DOI:https://doi.org/10.1021/bi00433a004· OSTI ID:5673222

Hoover, T R; Imperial, J; Ludden, P W; Shah, V K ^[1]

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1. Univ. of Wisconsin, Madison (USA)

When apodinitrogenase (lacking FeMo-co) was activated with FeMo-co synthesized in vitro in the presence of {sup 3}H-labeled homocitrate, label was incorporated into dinitrogenase. The physical association of he label with FeMo-co was demonstrated by reisolation and purification of the cofactor from dinitrogenase. The presence of homocitrate in FeMo-co was established by NMR analysis of the organic acid extracted from dinitrogenase. Quantitation of homocitrate in dinitrogenase showed it to be present at 1:1 ratio with molybdenum.

• OSTI ID: 5673222
• Journal Information: Biochemistry; (USA), Journal Name: Biochemistry; (USA) Vol. 28:7; ISSN 0006-2960; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BIOSYNTHESIS
CARBOXYLIC ACID SALTS
CITRATES
COENZYMES
ENZYMES
HYDROGEN COMPOUNDS
MAGNETIC RESONANCE
NITRO-GROUP DEHYDROGENASES
NITROGENASE
NUCLEAR MAGNETIC RESONANCE
OXIDOREDUCTASES
PURIFICATION
RESONANCE
SYNTHESIS
TRITIUM COMPOUNDS
UPTAKE