Expression of the human growth hormone variant gene in cultured fibroblasts and transgenic mice

Journal Article · Tue Nov 01 00:00:00 EST 1988 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.85.21.8241· OSTI ID:5673010

Selden, R F; Wagner, T E; Blethen, S; Yun, J S; Rowe, M E; Goodman, H M ^[1]

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1. Massachusetts General Hospital, Boston (USA)

The nucleotide sequence of the human growth hormone variant gene, one of the five members of the growth hormone gene family, predicts that it encodes a growth hormone-like protein. As a first step in determining whether this gene is functional in humans, the authors have expressed a mouse methallothionein I/human growth hormone variant fusion gene in mouse L cells and in transgenic mice. The growth hormone variant protein expressed in transiently transfected L cells is distinct from growth hormone itself with respect to reactivity with anti-growth hormone monoclonal antibodies, behavior during column chromatography, and isoelectric point. Transgenic mice expressing the growth hormone variant protein are 1.4- to 1.9-fold larger than nontransgenic controls, suggesting that the protein has growth-promoting properties.

• OSTI ID: 5673010
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 85:21; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GENES
DNA SEQUENCING
STH
RADIOIMMUNOASSAY
ANIMAL GROWTH
CELL CULTURES
FIBROBLASTS
HYBRIDIZATION
IODINE 125
MICE
PLASMIDS
PROTEINS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BIOASSAY
CELL CONSTITUENTS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
ELECTRON CAPTURE RADIOISOTOPES
GROWTH
HORMONES
IMMUNOASSAY
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PITUITARY HORMONES
RADIOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
RADIOISOTOPES
RODENTS
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
TRACER TECHNIQUES
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550201* - Biochemistry- Tracer Techniques