Human adrenodoxin reductase: Two mRNAs encoded by a single gene on chromosome 17cen yields q25 are expressed in steroidogenic tissues

Journal Article · Sat Oct 01 04:00:00 EDT 1988 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.85.19.7104· OSTI ID:5672836

Solish, S B; Picado-Leonard, J; Morel, Y; Kuhn, R W; Mohandas, T K; Hanukoglu, I; Miller, W L ^[1]

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1. Univ. of California, San Francisco (USA)

Adrenodoxin reductase is a mitochondrial flavoprotein that receives electrons from NADPH, thus initiating the electron-transport chain serving mitochondrial cytochromes P450. The authors have cloned and sequenced two human adrenodoxin reductase cDNAs that differ by the presence of six additional codons in the middle of one clone. The sequence in this region indicates that these six extra codons arise by alternative splicing of the pre-mRNA. Southern blot hybridization patterns of human genomic DNA cut with four restriction enzymes indicate that the human genome has only one gene for adrenodoxin reductase. Analysis of a panel of mouse-human somatic cell hybrids localized this gene to chromosome 17cen {yields} q25. The alternatively spliced mRNA containing the six extra codons represents 10-20% of all adrenodoxin reductase mRNA. The expression of the adrenodoxin reductase gene may be stimulated by pituitary tropic hormones acting through cAMP, but its response is quantitatively much less than the responses of P450scc and adrenodoxin.

• OSTI ID: 5672836
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 85:19; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

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59 BASIC BIOLOGICAL SCIENCES
ADRENAL GLANDS
AMINO ACID SEQUENCE
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOSYNTHESIS
BODY
CHROMOSOMES
DAYS LIVING RADIOISOTOPES
DNA
DNA SEQUENCING
ELECTRON TRANSFER
ENDOCRINE GLANDS
ENZYME ACTIVITY
ENZYMES
GENETIC MAPPING
GLANDS
HETEROCHROMOSOMES
HORMONES
HYBRIDIZATION
ISOTOPES
LIGHT NUCLEI
MAPPING
MESSENGER-RNA
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
RADIOISOTOPES
RECOMBINANT DNA
RNA
STEROID HORMONES
STRUCTURAL CHEMICAL ANALYSIS
SYNTHESIS