Neutrophil-derived oxidants mediate formyl-methionyl-leucyl-phenylalanine-induced increases in mucosal permeability in rats

Journal Article · Fri Sep 01 04:00:00 EDT 1989 · Gastroenterology; (USA)

OSTI ID:5672710

von Ritter, C; Grisham, M B; Hollwarth, M; Inauen, W; Granger, D N ^[1]

---

1. Louisiana State Univ. Medical Center, Shreveport (USA)

The effects of several free radical scavengers and antioxidant enzymes on neutrophil-mediated changes in mucosal permeability (measured using blood-to-lumen clearance of {sup 51}Cr-labeled ethylene-diaminetetraactate) were assessed using ileal loops perfused with N-formyl-methionyl-leucyl-phenylalanine (FMLP). Neither superoxide dismutase nor catalase reduced the FMLP-induced increase in mucosal permeability. However, manganese-loaded desferrioxamine (a superoxide dismutase mimetic), PZ51 (a glutathione peroxidase analogue), desferrioxamine (an iron chelator), or dimethylsulfoxide (a hydroxyl radical scavenger) significantly attenuated FMLP-induced mucosal damage. The results of our experiments indicate that neutrophilic oxidants are responsible for a major portion of the mucosal permeability changes induced by FMLP.

• OSTI ID: 5672710
• Journal Information: Gastroenterology; (USA), Journal Name: Gastroenterology; (USA) Vol. 97:3; ISSN GASTA; ISSN 0016-5085
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
560300 -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ABSORPTION
AMINO ACIDS
ANIMALS
ANTIOXIDANTS
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARBOXYLIC ACIDS
CATALASE
CHELATING AGENTS
CHROMIUM 51
CHROMIUM ISOTOPES
CLEARANCE
DIGESTIVE SYSTEM
EDTA
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
EVEN-ODD NUCLEI
GASTROINTESTINAL TRACT
INTERMEDIATE MASS NUCLEI
INTESTINAL ABSORPTION
INTESTINES
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
MAMMALS
MATERIALS
MEMBRANES
MUCOUS MEMBRANES
NEUTROPHILS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
PERMEABILITY
PEROXIDASES
PHENYLALANINE
RADICALS
RADIOISOTOPES
RATS
RODENTS
SMALL INTESTINE
SUPEROXIDE DISMUTASE
TRACER TECHNIQUES
UPTAKE
VERTEBRATES