Viability of the vascularly perfused, recirculating rat intestine and intestine-liver preparations

Journal Article · Tue Aug 01 00:00:00 EDT 1989 · American Journal of Physiology; (USA)

OSTI ID:5672408

Hirayama, H; Xu, X; Pang, K S ^[1]

---

1. Univ. of Toronto, Ontario (Canada)

Function and stability of vascularly perfused, recirculating in situ rat intestine (I) and intestine-liver (IL) preparations were evaluated in fasted and nonfasted rats because these techniques may be readily applied in drug metabolism studies. The rat intestine was perfused with blood medium (7.5 ml/min) via the superior mesenteric artery, with the venous outflow draining into the portal vein, which, together with hepatic arterial flow (2.5 ml/min), constituted the total blood flow (10 ml/min) to the liver. Maintenance of intestinal membrane integrity was observed. Rapid ({sup 14}C)glucose absorption against a concentration gradient and a lack of ({sup 3}H)-polyethylene glycol 4000 (PEG 4000, less than 4%) and Evans blue absorption by the recirculating I and IL preparations resulted after bolus injections of these markers into the pyloric end of the duodenum. Other indexes that revealed stable intestinal and liver functions were the following: preservation of reservoir perfusate volume, constancy in perfusion pressure, bile flow, and hemoglobin concentrations, evidence of intestinal glucose utilization and liver glucose production, and a lack of significant leakage of serum glutamic oxalic transaminase. The intestine and liver consumed oxygen at relatively constant rates, but the consumption rates for the fasted tissues (I or L) were significantly higher than those for nonfasted tissues. These results indicate that the vascularly perfused I and IL preparations were maintained in a viable and stable state for a 2-h perfusion period.

• OSTI ID: 5672408
• Journal Information: American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 257; ISSN 0002-9513; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ABSORPTION
ALCOHOLS
ALDEHYDES
ANIMALS
ARTERIES
BIOLOGICAL FUNCTIONS
BLOOD FLOW
BLOOD VESSELS
BODY
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARDIOVASCULAR SYSTEM
CELL CONSTITUENTS
CELL MEMBRANES
DIGESTIVE SYSTEM
ELEMENTS
FASTING
FUNCTIONS
GASTROINTESTINAL TRACT
GLANDS
GLUCOSE
GLYCOLS
HEXOSES
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
INTESTINAL ABSORPTION
INTESTINES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIVER
MAMMALS
MEMBRANES
METABOLISM
MONOSACCHARIDES
NONMETALS
ORGANIC COMPOUNDS
ORGANIC POLYMERS
ORGANS
OXYGEN
PERFUSED ORGANS
POLYETHYLENE GLYCOLS
POLYMERS
RATS
RODENTS
SACCHARIDES
SMALL INTESTINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
UPTAKE
VERTEBRATES