Magnetic resonance spectral characterization of the heme active site of Coprinus cinereus peroxidase

Journal Article · Tue Apr 18 00:00:00 EDT 1989 · Biochemistry; (USA)

DOI:https://doi.org/10.1021/bi00434a032· OSTI ID:5672036

Lukat, G S; Rodgers, K R; Jabro, M N; Goff, H M ^[1]

---

1. Univ. of Iowa, Iowa City (USA)

Examination of the peroxidase isolated from the inkcap Basidiomycete Coprinus cinereus shows that the 42,000-dalton enzyme contains a protoheme IX prosthetic group. Reactivity assays and the electronic absorption spectra of native Coprinus peroxidase and several of its ligand complexes indicate that this enzyme has characteristics similar to those reported for horseradish peroxidase. In this paper, the authors characterize the H{sub 2}O{sub 2}-oxidized forms of Coprinus peroxidase compounds I, II, and III by electronic absorption and magnetic resonance spectroscopies. Electron paramagnetic resonance (EPR) and nuclear magnetic resonance (NMR) studies of this Coprinus peroxidase indicate the presence of high-spin Fe(III) in the native protein and a number of differences between the heme site of Coprinus peroxidase and horseradish peroxidase. Carbon-13 (of the ferrous CO adduct) and nitrogen-15 (of the cyanide complex) NMR studies together with proton NMR studies of the native and cyanide-complexed Caprinus peroxidase are consistent with coordination of a proximal histidine ligand. The EPR spectrum of the ferrous NO complex is also reported. Protein reconstitution with deuterated hemin has facilitated the assignment of the heme methyl resonances in the proton NMR spectrum.

• OSTI ID: 5672036
• Journal Information: Biochemistry; (USA), Journal Name: Biochemistry; (USA) Vol. 28:8; ISSN 0006-2960; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ABSORPTION SPECTRA
BARYONS
CARBON 13
CARBON ISOTOPES
CHEMICAL SHIFT
ELECTRON SPIN RESONANCE
ELEMENTARY PARTICLES
ENZYME ACTIVITY
ENZYMES
EVEN-ODD NUCLEI
FERMIONS
FUNGI
HADRONS
HEAVY WATER
HYDROGEN COMPOUNDS
ISOTOPES
LIGANDS
LIGHT NUCLEI
MAGNETIC RESONANCE
MOLECULAR STRUCTURE
NITROGEN 15
NITROGEN ISOTOPES
NUCLEAR MAGNETIC RESONANCE
NUCLEI
NUCLEONS
ODD-EVEN NUCLEI
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PEROXIDASES
PH VALUE
PLANTS
PROTONS
RESONANCE
SPECTRA
STABLE ISOTOPES
WATER