Possible role of regional superoxide dismutase activity and lipid peroxide levels in cadmium neurotoxicity: in vivo and in vitro studies in growing rats

Journal Article · Mon Nov 09 00:00:00 EST 1987 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(87)90518-2· OSTI ID:5664161

Shukla, G S; Hussain, T; Chandra, S V

Cd/sup 2 +/ (0.4 mg/kg) administration to growing rats (45 +/- 5 g) intraperitoneally, daily for 30 days was found to decrease the activity of superoxide dismutase (SOD) in all the brain regions, except hippocampus. The concentrations of lipid peroxides were significantly elevated in the cerebellum, cerebral cortex, corpus striatum and midbrain. A 100% inhibition in SOD activity was observed by 14 ..mu..M and 50 ..mu..M of Cd/sup 2 +/ in bovine blood and rat brain preparations, respectively. Cadmium-induces strong inhibitory effect on brain and purified bovine blood SOD suggested a direct effect of the metal on enzyme molecule. Furthermore, in vitro addition of a wide range of Cd/sup 2 +/ (1-100 ..mu..M) increased the rate of lipid peroxidation (LPO) reactions in fresh brain homogenate, however, did not affect boiled homogenate. The studies on LPO in reconstituted homogenate resulting from mixing of fresh and/or heated different subcellular fractions indicated the presence of some heat-labile Cd/sup 2 +/-sensitive factor in 15,000 x g pellet fraction. It is suggested that Cd/sup 2 +/ directly and indirectly through inhibition of SOD, increases the LOP of cell membranes and thus produces damage to the associated physiological functions leading to central nervous dysfunctions. 53 references, 1 figure, 2 tables.

• Research Organization: Industrial Toxicology Research Centre, Locknow, India
• OSTI ID: 5664161
• Journal Information: Life Sci.; (United States), Vol. 41:19
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CADMIUM
TOXICITY
LIPIDS
BIOSYNTHESIS
SUPEROXIDE DISMUTASE
ENZYME ACTIVITY
CENTRAL NERVOUS SYSTEM
EXPERIMENTAL DATA
HOMOGENATES
IN VITRO
IN VIVO
INHIBITION
INTRAPERITONEAL INJECTION
RATS
ANIMALS
DATA
ELEMENTS
ENZYMES
INFORMATION
INJECTION
INTAKE
MAMMALS
METALS
NERVOUS SYSTEM
NUMERICAL DATA
ORGANIC COMPOUNDS
OXIDOREDUCTASES
RODENTS
SYNTHESIS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology