Biological breakdown of RDX in slurry reactors proceeds with multiple kinetically distinguishable paths

Journal Article · Wed Nov 05 04:00:00 EST 1997 · Biotechnology and Bioengineering

DOI:https://doi.org/10.1002/(SICI)1097-0290(19971105)56:3<258::AID-BIT3>3.3.CO;2-Z· OSTI ID:566363

Young, D M; Ogden, K L ^[1]; Kitts, C L ^[2]; Unkefer, P J ^[3]

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1. Univ. of Arizona, Tucson (United States). Dept. of Chemical and Environmental Engineering
2. California Polytechnic State Univ., San Luis Obispo, CA (United States). Biological Sciences Dept.
3. Los Alamos National Lab., NM (United States). Chemical Science and Technology Div.

Biotransformation of RDX (hexahydro-1,3,5-trinitro-1,3,5-triazine) in slurry reactors was studied to determine the importance of supplementation of known biodegraders and the type of nutrient source required. Although addition of bacteria to the system increased the biotransformation rates, the increase may not justify the additional work and cost needed to grow the organisms in a laboratory and mix them into the soil. An inexpensive, rich nutrient source, corn steep liquor, was shown to provide sufficient nutrients to allow for the cometabolic biotransformation of RDX. The rate of RDX transformation was not constant throughout the course of the experiment due to the heterogeneous microbial population. Three kinetically distinct phases were observed. Regardless of the process, RDX biotransformation in slurry reactors was reaction rate limited under the test conditions. Model simulations based on experimental results demonstrate that, at cell densities of 5 g/L, bioremediation of RDX-contaminated soil is an attractive clean-up alternative.

• OSTI ID: 566363
• Journal Information: Biotechnology and Bioengineering, Journal Name: Biotechnology and Bioengineering Journal Issue: 3 Vol. 56; ISSN BIBIAU; ISSN 0006-3592
• Country of Publication: United States
• Language: English

Related Subjects

45 MILITARY TECHNOLOGY, WEAPONRY, AND NATIONAL DEFENSE
54 ENVIRONMENTAL SCIENCES
AMMUNITION
BIODEGRADATION
BIOREACTORS
NITRO COMPOUNDS
PERFORMANCE TESTING
REMEDIAL ACTION
SLURRIES
SOILS