Carbon-13 relaxation behavior in multiply enriched systems: (90%-1,2-/sup 13/C/sub 2/-Gly/sup 6/)bradykinin
- Los Alamos National Lab., NM
The relaxation behavior of macromolecules multiply enriched in /sup 13/C has been investigated theoretically, and results for (90%-1,2-/sup 13/C/sub 2/-Gly/sup 6/)bradykinin have been interpreted on the basis of this theory. In the case of (90%-1, 2-/sup 13/C/sub 2/-Gly/sub 6/)bradykinin, the carbonyl doublet is broadened relative to the center resonance due to the short Gly methylene T/sub 1/. The resulting spectrum can be analyzed quantitatively to obtain the Gly methylene T/sub 1/. The method therefore provides a quick (single spectrum) means for obtaining T/sub 1/ information. Studies of the labeled bradykinin have been carried out in solvents of varying viscosity and the data analyzed by using the theory presented. The relaxation behavior of peptides in high viscosities is found to differ from that of globular proteins for which segmental motion is estimated to be of lower amplitude and consequently less significant as a relaxation mechanism.
- OSTI ID:
- 5658347
- Journal Information:
- Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 21:3; ISSN BICHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
400700* -- Radiochemistry & Nuclear Chemistry
ALCOHOLS
BRADYKININ
CARBON 13
CARBON ISOTOPES
DIPOLES
DOUBLE LABELLING
EVEN-ODD NUCLEI
GLYCEROL
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
KININS
LABELLED COMPOUNDS
LABELLING
LIGHT NUCLEI
MULTIPOLES
NUCLEI
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PEPTIDES
PROTEINS
RELAXATION
SOLVENTS
SPIN-LATTICE RELAXATION
STABLE ISOTOPES
TRACER TECHNIQUES
VISCOSITY
WATER