Resistance of a cultured fish cell line (CAF-MM1) to. gamma. irradiation
Fish are generally more resistant to whole-body ionizing radiation than mammals. To study the radiosensitivity of fish in vitro, CAF-MM1 cells derived from the fin of the goldfish, Carassius auratus, were used. The survival parameters of CAF-MM1 obtained after ..gamma.. irradiation at 26/sup 0/C were 325 rad for D/sub o/, 975 rad for Dq, and 15 for n. No mammalian cell line with such a low sensitivity in the presence of O/sub 2/ has been reported. It was found that the large initial shoulder of the survival curve was paralleled by substantial repair of sublethal damage as evidenced by split-dose experiments. This low sensitivity to ..gamma.. irradiation did not change upon the administration of caffeine or postirradiation illumination, although these treatments were effective after uv irradiation. The decrease in the mitotic index in CAF-MM1 occurred immediately after irradiation, and it recovered within a very short time. This indicated that the duration of G2 arrest was shorter than that observed in mammalian cells. The data also suggest that the resistance of fish to whole-body irradiation is attributable to resistance at the cellular level.
- OSTI ID:
- 5654281
- Journal Information:
- Radiat. Res.; (United States), Vol. 89:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
GOLDFISH
BIOLOGICAL RADIATION EFFECTS
ANIMAL CELLS
CAFFEINE
CELL CULTURES
GAMMA RADIATION
IN VITRO
OXYGEN
RADIATION DOSES
RADIOSENSITIVITY
SURVIVAL CURVES
ULTRAVIOLET RADIATION
WHOLE-BODY IRRADIATION
ANALEPTICS
ANIMALS
AQUATIC ORGANISMS
BIOLOGICAL EFFECTS
CENTRAL NERVOUS SYSTEM AGENTS
DOSES
DRUGS
ELECTROMAGNETIC RADIATION
ELEMENTS
EXTERNAL IRRADIATION
FISHES
HETEROCYCLIC COMPOUNDS
IONIZING RADIATIONS
IRRADIATION
NONMETALS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PURINES
RADIATION EFFECTS
RADIATIONS
VERTEBRATES
XANTHINES
560152* - Radiation Effects on Animals- Animals
560121 - Radiation Effects on Cells- External Source- (-1987)