Inhibition of mixed leukocyte culture responses in cadmium-treated mice

Journal Article · Thu Apr 01 04:00:00 EST 1982 · Environ. Res.; (United States)

DOI:https://doi.org/10.1016/0013-9351(82)90097-4· OSTI ID:5653946

Bozelka, B E ^[1]; Burkholder, P M

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1. Tulane Univ. School of Medicine, New Orleans, LA

Previous studies have documented inhibition of antibody-mediated immunity by exposure of experimental animals to heavy metals. To date, very little is known of possible effects of heavy metal ions cellular-mediated immunity. The mixed leukocyte culture (MLC) assay was used in this study to evaluate the effects on lymphocyte blastogenic response of chronic exposure to mice to cadmium (2 mg CdCl/sub 2//kg body wt/day). Spleen cells from two strains of mice treated with CdCl/sub 2/ were unable to respond as effectively as control normal spleen cells to stimulation with allogeneic leukocytes. Furthermore, X-irradiated splenocytes from the cadmium-stressed animals were less effective than splenocytes from control normal mice as stimulator cells in the MLC reaction. This comparative decrease in T-lymphocyte reactivity could not be attributed to loss of lymphocyte viability or to decrease in ability of lymphocytes from cadmium-treated animals to incorporate tritiated thymidine. Although spleen cells from cadmium-intoxicated mice responded poorly to stimulation with allogeneic leukocytes, they were capable of responding to a mitogenic stimulus. Possible explanations for these observations are discussed.

• OSTI ID: 5653946
• Journal Information: Environ. Res.; (United States), Journal Name: Environ. Res.; (United States) Vol. 27:2; ISSN ENVRA
• Country of Publication: United States
• Language: English

Related Subjects

560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOASSAY
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CADMIUM
CELL CULTURES
CHRONIC EXPOSURE
CHRONIC INTAKE
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
ELEMENTS
IMMUNOSUPPRESSION
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INTAKE
IONIZING RADIATIONS
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MATERIALS
METALS
MICE
RADIATIONS
RODENTS
SOMATIC CELLS
SPLEEN CELLS
VERTEBRATES
X RADIATION