Indium-111-radiolabeled guinea pig peripheral leukocytes. In vivo distribution and response to leukotriene B4
The preparation of indium-111 tropolonate-radiolabeled guinea pig peripheral mixed white cells (greater than 80% neutrophils) is described. Autologous rather than homologous cells are required to provide a population of labeled, functional cells on reintroduction to the animals. Surgery has been shown to result in a profound neutropenia from which the animals must recover before removal of blood for cell preparation. The response of radiolabeled cells parallels that of the unlabeled cell population to a chemotaxin, leukotriene B4. This material causes a profound neutropenia of rapid onset accompanied by a parallel fall in blood radioactivity. The fall in circulating radiolabel is accompanied by an increase in radioactivity in the thoracic region. These changes have been monitored externally using an automated isotope monitoring system.
- Research Organization:
- Eli Lilly and Co., Surrey, England
- OSTI ID:
- 5653657
- Journal Information:
- J. Pharmacol. Methods; (United States), Vol. 18:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ARACHIDONIC ACID
BIOLOGICAL EFFECTS
LEUKOCYTES
SAMPLE PREPARATION
TISSUE DISTRIBUTION
DOSE-RESPONSE RELATIONSHIPS
GUINEA PIGS
INDIUM 111
SURGERY
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
DISTRIBUTION
ELECTRON CAPTURE RADIOISOTOPES
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MAMMALS
MATERIALS
MEDICINE
MINUTES LIVING RADIOISOTOPES
MONOCARBOXYLIC ACIDS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
RADIOISOTOPES
RODENTS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques