Effect of tungstate on nitrate assimilation in higher plant tissues

Journal Article · Tue Dec 31 23:00:00 EST 1968 · Plant Physiol.; (United States)

DOI:https://doi.org/10.1104/pp.44.8.1197· OSTI ID:5651031

Heimer, Y M; Wray, J L; Filner, P

Tungstate is not only a competitive inhibitor of molybdate uptake and utilization in Azotobacter vinelandii, but also inhibits growth of this organism when nitrate or nitrogen is the sole nitrogen source. The effect of tungstate on the formation of active nitrate reductase in suspension cultures of tobacco XD cells and intact barley shoots was examined. In both cases tungstate prevented the formation of active enzyme. Because of its broad biological spectrum of action, tungstate may be used to selectively prevent nitrate reduction. It will now be possible to examine the regulation of nitrate uptake and of other steps of the nitrate assimilation pathway in higher plants without the complications introduced by the functioning of the pathway. 13 references, 2 figures, 2 table.

• Research Organization: Michigan State Univ., East Lansing
• OSTI ID: 5651031
• Journal Information: Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 44; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

560303* -- Chemicals Metabolism & Toxicology-- Plants-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BARLEY
BIOLOGICAL EFFECTS
BIOLOGICAL PATHWAYS
BIOSYNTHESIS
CELL CULTURES
CEREALS
CHEMICAL REACTIONS
ELEMENTS
ENZYMES
GRASS
INHIBITION
METALS
MOLYBDENUM
NICOTIANA
NITRATES
NITROGEN COMPOUNDS
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PLANTS
REDUCTION
SYNTHESIS
TRANSITION ELEMENTS
TUNGSTATES
UPTAKE