Cholinergic ligand interactions with acetylcholine receptor proteins and solvent interactions with N,N-dialkylnicotinamides
A dual-chambered flow dialysis nuclear counting apparatus was used to monitor cholinergic ligand induced displacement of {sup 155}Eu{sup 3+} from acetylcholine receptor proteins. Acetylcholine, nicotine and carbamylcholine induced similar rates of displacement of {sup 155}Eu{sup 3+} probes of calcium binding sites in receptor proteins from wild type Drosophila melanogaster and Torpedo californica. The receptor isolated from a nicotine resistant strain of Drosophila melanogaster displayed an altered dependency of cholinergic ligand induced cation displacement with respect to the other two receptor proteins. Both Drosophila strains' solubilized receptor proteins migrated as three bands of molecular weights 68,000, 66,000, and 60,000 on denaturing polyacrylamide gels. Carbon-13 NMR techniques were employed to examine the effects of solvent environment on rotational energy barriers in a series of molecules related to the analeptic, nikethamide: N,N-dimethylnicotinamide, 1-nicotinoyl piperidine, and N,N-dipropylnicotinamide.
- Research Organization:
- Clark Univ., Worcester, MA (USA)
- OSTI ID:
- 5649303
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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ACETYLCHOLINE
RECEPTORS
AMIDES
BIOCHEMICAL REACTION KINETICS
PIPERIDINES
SOLVENTS
CARBON 13
DROSOPHILA
ELECTROPHORESIS
EUROPIUM 155
FISHES
LIGANDS
MOLECULAR WEIGHT
NMR SPECTRA
TRACER TECHNIQUES
AMINES
AMMONIUM COMPOUNDS
ANIMALS
AQUATIC ORGANISMS
ARTHROPODS
AUTONOMIC NERVOUS SYSTEM AGENTS
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBON ISOTOPES
DIPTERA
DRUGS
ESTERS
EUROPIUM ISOTOPES
EVEN-ODD NUCLEI
FLIES
FRUIT FLIES
HETEROCYCLIC COMPOUNDS
INSECTS
INTERMEDIATE MASS NUCLEI
INVERTEBRATES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
MEMBRANE PROTEINS
NEUROREGULATORS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASYMPATHOMIMETICS
PROTEINS
PYRIDINES
QUATERNARY COMPOUNDS
RADIOISOTOPES
RARE EARTH ISOTOPES
RARE EARTH NUCLEI
REACTION KINETICS
SPECTRA
STABLE ISOTOPES
VERTEBRATES
YEARS LIVING RADIOISOTOPES
550201* - Biochemistry- Tracer Techniques