Bacteriochlorophyll protein structure - studies with polarized light and triplet state electron paramagnetic resonance
Technical Report
·
OSTI ID:5645588
Light harverting bacteriochlorophyll-protein complexes from Rhodopseudomonas sphaeroides 2.4.1 (B800 + 850) and R-26 (LH-R26) mutant are solubiliized in SDS and imbedded in polyvinyl alcohol. Stretching induces orientation, and the linear dichroism of visible and near infrared absorption is analyzed. Based on a simple model, angles between the particle axis system and the transition dipole moments are found. Reaction centers of the photosynthetic bacterium Rhodopseudomonas sphaeroides R-26, give rise to large triplet state EPR signals upon illumination at low temperature (11 K). Utilizing monochromatic polarized light to generate the EPR spectra (magnetophotoselection), it is shown that the intensities of the observed triplet signals are strongly dependent upon the wavelength and polarization direction of the excitation. Triplet states of carotenoids have been detected by EPR and are reported here for the first time. The systems in which carotenoid triplets are observed include cells of photosynthetic bacteria, isolated bacteriochlorophyll-protein complexes, and detergent micelles which contain ..beta..-carotene. The carotenoid triplet state is detected in reaction centers of Rhodopseudomonas sphaeroides 2.4.1 (wild type), which contain the carotenoid spheroidene.
- Research Organization:
- California Univ., Berkeley (USA). Lawrence Berkeley Lab.
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 5645588
- Report Number(s):
- LBL-10560
- Country of Publication:
- United States
- Language:
- English
Similar Records
Electron paramagnetic resonance detection of carotenoid triplet states
Spectral diffusion and [sup 14]N quadrupole splittings in absorption detected magnetic resonance hole burning spectra of photosynthetic reaction centers
Correlation of paramagnetic states and molecular structure in bacterial photosynthetic reaction centers: The symmetry of the primary electron donor in Rhodopseudomonas viridis and Rhodobacter sphaeroides R-26
Journal Article
·
Wed Jul 16 00:00:00 EDT 1980
· J. Am. Chem. Soc.; (United States)
·
OSTI ID:6776119
Spectral diffusion and [sup 14]N quadrupole splittings in absorption detected magnetic resonance hole burning spectra of photosynthetic reaction centers
Journal Article
·
Thu Mar 31 23:00:00 EST 1994
· Journal of Chemical Physics; (United States)
·
OSTI ID:5212316
Correlation of paramagnetic states and molecular structure in bacterial photosynthetic reaction centers: The symmetry of the primary electron donor in Rhodopseudomonas viridis and Rhodobacter sphaeroides R-26
Journal Article
·
Thu Jun 01 00:00:00 EDT 1989
· Proceedings of the National Academy of Sciences of the United States of America; (USA)
·
OSTI ID:5441618
Related Subjects
550200* -- Biochemistry
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
CARBOXYLIC ACIDS
CHLOROPHYLL
ELECTRON SPIN RESONANCE
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
MAGNETIC RESONANCE
MICROORGANISMS
MOLECULAR STRUCTURE
MULTIPLETS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHYTOCHROMES
PIGMENTS
PORPHYRINS
RESONANCE
RHODOPSEUDOMONAS
TRIPLETS
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
CARBOXYLIC ACIDS
CHLOROPHYLL
ELECTRON SPIN RESONANCE
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
MAGNETIC RESONANCE
MICROORGANISMS
MOLECULAR STRUCTURE
MULTIPLETS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHYTOCHROMES
PIGMENTS
PORPHYRINS
RESONANCE
RHODOPSEUDOMONAS
TRIPLETS