Cell surfaces in plant-microorganism interactions. I. A structural investigation of cell wall hydroxyproline-rich glycoproteins which accumulate in fungus-infected plants

Journal Article · Wed Aug 01 00:00:00 EDT 1979 · Plant Physiol.; (United States)

DOI:https://doi.org/10.1104/pp.64.2.314· OSTI ID:5644474

Esquerre-Tugaye, M T ^[1]; Lamport, D T.A.

---

1. Universite Paul Sabatier, Toulouse, France

Infection of muskmelon Cucumis melo seedlings by the fungus Colletotrichum lagenarium causes a 10-fold increase in the amount of cell wall hydroxyproline-rich glycoprotein. Evidence for this increase was provided by studying two specific markers of this glycoprotein, namely hydroxyproline and glycosylated serine. The lability of the O-glycosidic linkage of wall-bound glycosylated serine in the presence of hydrazine was used to determine the amount of serine which is glycosylated. A large increase in the hydroxyproline content of infected plants is shown, but the ratios of glycosylated serine to hydroxyproline are similar in healthy and infected plants. As far as these markers are concerned, the hydroxyproline-rich glycoproteins secreted into the wall as a result of the disease are similar to those of healthy plants. In addition, the extent of glycosylation of the wall serine, in both healthy and infected plants, decreases as the plant ages. Serine- and hydroxyproline-rich (glyco)peptides were also isolated after trypsinolysis of the wall. These (glyco)peptides include the galactosyl-containing pentapeptide, serine-hydroxyproline. This pentapeptide is characteristic of cell wall protein.

• OSTI ID: 5644474
• Journal Information: Plant Physiol.; (United States), Vol. 64:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PLANT CELLS
MORPHOLOGY
CELL WALL
FUNGI
GLUCOPROTEINS
HYDROXYPROLINE
METABOLISM
MICROORGANISMS
PLANTS
PROTEINS
SERINE
AMINES
AMINO ACIDS
AZOLES
CARBOHYDRATES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROXY ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRROLES
PYRROLIDINES
SACCHARIDES
550800* - Morphology
550200 - Biochemistry
550500 - Metabolism