Effect of oxotremorine, physostigmine, and scopolamine on brain acetylcholine synthesis: a study using HPLC

Journal Article · Wed Oct 31 23:00:00 EST 1990 · Neurochemical Research; (USA)

DOI:https://doi.org/10.1007/BF01101710· OSTI ID:5643001

Bertrand, N; Beley, A ^[1]

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1. Laboratoire de Pharmacodynamie et de Physiologie Pharmaceutique, Faculte de Pharmacie, Dijon, (France)

The synthesis rate of brain acetylcholine (ACh) was estimated in mice following i.v. administration of ({sup 3}H)choline (Ch). The measurements were performed 1 min after the tracer injection, using the ({sup 3}H)ACh/({sup 3}H)Ch specific radioactivity ratio as an index of ACh synthesis rate. Endogenous and labeled Ch and ACh were quantified using HPLC methodology. Oxotremorine and physostigmine (0.5 mg/kg, i.p.) increased the steady state concentration of brain ACh by + 130% and 84%, respectively and of Ch by + 60% (oxotremorine); they decreased ACh synthesis by 62 and 55%, respectively. By contrast, scopolamine (0.7 mg/kg, i.p.) decreased the cerebral content of Ch by - 26% and of ACh by - 23% without enhancing the synthesis of ACh. The results show the utility of HPLC methodology in the investigation of ACh turnover.

• OSTI ID: 5643001
• Journal Information: Neurochemical Research; (USA), Journal Name: Neurochemical Research; (USA) Vol. 15:11; ISSN 0364-3190; ISSN NERED
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACETYLCHOLINE
ALCOHOLS
AMINES
AMMONIUM COMPOUNDS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOCHEMICAL REACTION KINETICS
BIOCHEMISTRY
BIOSYNTHESIS
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
CHEMISTRY
CHOLINE
CHROMATOGRAPHY
DRUGS
ESTERS
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LIPOTROPIC FACTORS
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MICE
NERVOUS SYSTEM
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANS
PARASYMPATHOMIMETICS
QUATERNARY COMPOUNDS
REACTION KINETICS
RODENTS
SEPARATION PROCESSES
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES