Sea urchin sperm antigens mediating the acrosome reaction

Thesis/Dissertation · Thu Jan 01 04:00:00 EST 1987

OSTI ID:5642962

Trimmer, J S

The study of sea urchin sperm antigens mediating the acrosome reactions (AR) has been undertaken. Monoclonal antibodies (mAbs) have been isolated reacting with a number of sperm surface antigens. These mAbs have been used in functional assays to attempt to infer the roles of these proteins in the induction of the AR. These mAbs have also been used to isolate protein for biochemical characterization and reconstitution studies. mAbs reacting with a 210 kD protein of the sea urchin sperm plasma membrane have been used to identify this protein as playing a role in the regulation of ion fluxes during the induction of the AR. mAbs reacting with certain extracellular regions inhibit the induction of: the AR, the long duration {sup 45}Ca{sup 2+} uptake into the mitochondrion, and H{sup +} efflux. Addition of these same mAbs, however, induces an increase in sperm (Ca{sup 2+}){sub i} to levels much higher than those induced by FSG, as monitored by the fluorescent Ca{sup 2+} indicators fura 2 and indo 1. This (Ca{sup 2+}){sub i} increase occurs without an increase in pH{sub i}, and thus allows for the first time the analysis of the effects of increasing sperm (Ca{sup 2+}){sub i} ion the absence of increased pH{sub i}.

• Research Organization: California Univ., San Diego, CA (USA)
• OSTI ID: 5642962
• Country of Publication: United States
• Language: English

Related Subjects

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59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METAL COMPOUNDS
ANIMALS
ANTIBODIES
ANTIGEN-ANTIBODY REACTIONS
ANTIGENS
AQUATIC ORGANISMS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL FUNCTIONS
CALCIUM 45
CALCIUM COMPOUNDS
CALCIUM ISOTOPES
CELL CONSTITUENTS
CELL MEMBRANES
DAYS LIVING RADIOISOTOPES
ECHINODERMS
EVEN-ODD NUCLEI
FUNCTIONS
GAMETES
GERM CELLS
INTERMEDIATE MASS NUCLEI
INVERTEBRATES
ISOTOPE APPLICATIONS
ISOTOPES
MEMBRANE TRANSPORT
MEMBRANES
MITOCHONDRIA
MONOCLONAL ANTIBODIES
NUCLEI
ORGANOIDS
RADIOISOTOPES
SEA URCHINS
SPERMATOZOA
TRACER TECHNIQUES