Receptor binding sites for atrial natriuretic factor are expressed by brown adipose tissue

Journal Article · Thu Sep 01 00:00:00 EDT 1988 · Peptides (Fayetteville, New York); (USA)

DOI:https://doi.org/10.1016/0196-9781(88)90083-6· OSTI ID:5641806

Bacay, A C; Mantyh, C R; Vigna, S R; Mantyh, P W ^[1]

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1. Wadsworth VA Medical Center, Los Angeles, CA (USA)

To explore the possibility that atrial natriuretic factor (ANF) is involved in thermoregulation we used quantitative receptor autoradiography and homogenate receptor binding assays to identify ANF bindings sites in neonatal rat and sheep brown adipose tissue, respectively. Using quantitative receptor autoradiography were were able to localize high levels of specific binding sites for {sup 125}I-rat ANF in neonatal rat brown adipose tissue. Homogenate binding assays on sheep brown fat demonstrated that the radioligand was binding to the membrane fraction and that the specific binding was not due to a lipophilic interaction between {sup 125}I-rat ANF and brown fat. Specific binding of {sup 125}I-rat ANF to the membranes of brown fat cells was inhibited by unlabeled rat ANF with a Ki of 8.0 x 10(-9) M, but not by unrelated peptides. These studies demonstrate that brown fat cells express high levels of ANF receptor binding sites in neonatal rat and sheep and suggest that ANF may play a role in thermoregulation.

• OSTI ID: 5641806
• Journal Information: Peptides (Fayetteville, New York); (USA), Journal Name: Peptides (Fayetteville, New York); (USA) Vol. 9:5; ISSN PPTDD; ISSN 0196-9781
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADIPOSE TISSUE
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BODY
CELL CONSTITUENTS
CELL MEMBRANES
CONNECTIVE TISSUE
CONNECTIVE TISSUE CELLS
CONTROL
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
ELECTRON CAPTURE RADIOISOTOPES
FAT CELLS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
KINETICS
LIGANDS
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
NEONATES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PEPTIDES
PROTEINS
RADIOISOTOPES
RATS
REACTION KINETICS
RECEPTORS
RODENTS
RUMINANTS
SHEEP
SOMATIC CELLS
SPECIFICITY
TEMPERATURE CONTROL
THERMOREGULATION
TISSUES
VERTEBRATES