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Purification and reconstitution of chloride channels from kidney and trachea

Journal Article · · Science (Washington, D.C.); (USA)
; ; ; ; ;  [1]
  1. Columbia Univ., New York, NY (USA)
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Chloride channels mediate absorption and secretion of fluid in epithelia, and the regulation of these channels is now known to be defective in cystic fibrosis. Indanyl-oxyacetic acid 94 (IAA-94) is a high-affinity ligand for the chloride channel, and an affinity resin based on that structure was developed. Solubilized proteins from kidney and trachea membranes were applied to the affinity matrix, and four proteins with apparent molecular masses of 97, 64, 40, and 27 kilodaltons were eluted from the column by excess IAA-94. A potential-dependent {sup 36}Cl- uptake was observed after reconstituting these proteins into liposomes. Three types of chloride channels with single-channel conductances of 26, 100, and 400 picosiemens were observed after fusion of these liposomes with planar lipid bilayers. Similar types of chloride channels have been observed in epithelia.

OSTI ID:
5641408
Journal Information:
Science (Washington, D.C.); (USA), Journal Name: Science (Washington, D.C.); (USA) Vol. 244:4911; ISSN SCIEA; ISSN 0036-8075
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
550901 -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL TISSUES
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CATTLE
CELL CONSTITUENTS
CELL MEMBRANES
CHLORIDES
CHLORINE 36
CHLORINE COMPOUNDS
CHLORINE ISOTOPES
CHROMATOGRAPHY
CONGENITAL DISEASES
DISEASES
DOMESTIC ANIMALS
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
ELECTROPHYSIOLOGY
EPITHELIUM
FRACTIONATION
HALIDES
HALOGEN COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
LIGHT NUCLEI
LIPOSOMES
MAMMALS
MEMBRANE PROTEINS
MEMBRANE TRANSPORT
MEMBRANES
MOLECULAR WEIGHT
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
PHYSIOLOGY
PIGMENTS
PROTEINS
RADIOISOTOPES
RESPIRATORY SYSTEM
RHODOPSIN
RUMINANTS
SEPARATION PROCESSES
TISSUES
TRACER TECHNIQUES
TRACHEA
VALINOMYCIN
VERTEBRATES
YEARS LIVING RADIOISOTOPES