Chromosomal changes in cultured human epithelial cells transformed by low- and high-LET radiation
For a better assessment of radiation risk in space, an understanding of the responses of human cells, especially the epithelial cells, to low- and high-LET radiation is essential. In our laboratory, we have successfully developed techniques to study the neoplastic transformation of two human epithelial cell systems by ionizing radiation. These cell systems are human mammary epithelial cells (H184B5) and human epidermal keratinocytes (HEK). Both cell lines are immortal, anchorage dependent for growth, and nontumorigenic in athymic nude nice. Neoplastic transformation was achieved by irradiation cells successively. Our results showed that radiogenic cell transformation is a multistep process and that a single exposure of ionizing radiation can cause only one step of transformation. It requires, therefore, multihits to make human epithelial cells fully tumorigenic. Using a simple karyotyping method, we did chromosome analysis with cells cloned at various stages of transformation. We found no consistent large terminal deletion of chromosomes in radiation-induced transformants. Some changes of total number of chromosomes, however, were observed in the transformed cells. These transformants provide an unique opportunity for further genetic studies at a molecular level. 15 refs., 9 figs., 2 tabs.
- Research Organization:
- Lawrence Berkeley Lab., CA (USA)
- Sponsoring Organization:
- National Aeronautic and Space Administration (NASA); National Aeronautics and Space Administration, Washington, DC (USA)
- DOE Contract Number:
- AC03-76SF00098
- OSTI ID:
- 5639531
- Report Number(s):
- LBL-30024; CONF-9006220-9; ON: DE91013772; CNN: T139M
- Resource Relation:
- Conference: COSPAR plenary conference, The Hague (Netherlands), 25 Jun - 6 Jul 1990
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ANIMAL CELLS
IRRADIATION
CELL TRANSFORMATIONS
RADIOINDUCTION
CHROMOSOMAL ABERRATIONS
IONIZING RADIATIONS
CARCINOGENESIS
BENZOPYRENE
CELL CULTURES
EPITHELIUM
IN VITRO
LET
MAMMARY GLANDS
ANIMAL TISSUES
AROMATICS
BODY
CONDENSED AROMATICS
ENERGY TRANSFER
GLANDS
HYDROCARBONS
MUTATIONS
ORGANIC COMPOUNDS
ORGANS
PATHOGENESIS
RADIATIONS
TISSUES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture