Migration into an in vitro experimental wound: a comparison of porcine aortic endothelial and smooth muscle cells and the effect of culture irradiation
Journal Article
·
· Am. J. Pathol.; (United States)
OSTI ID:5639474
The purpose of this study was to compare the group-cell migration characteristics of endothelial cells (ECs) and smooth muscle cells (SMCs) derived from the same source, the porcine thoracic aorta, as they moved into an experimental in vitro wound. The authors characterized migration by measuring two aspects of the migrating cells: the number of free cells in the wound and the distance of migration of the sheet of cells at the wound edge. The quantitative data showed that ECs migrated into the wound as a sheet of cells, while SMCs migrated as free single cells. In addition, since irradiated cells have been used to study cell migration and since the irradiated cells do undergo some shape changes, the distribution of the cytoskeletal microfilament fibres was compared in migrating irradiated and nonirradiated cells in order to see whether this feature of cell migration was different. Irradiated and nonirradiated migrating ECs showed a strikingly different pattern in the orientation of microfilament bundles when studied by immunofluorescence microscopy with antiserums to myosin and tropomyosin.
- OSTI ID:
- 5639474
- Journal Information:
- Am. J. Pathol.; (United States), Journal Name: Am. J. Pathol.; (United States) Vol. 103:2; ISSN AJPAA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Phenotypic heterogeneity influences the behavior of rat aortic smooth muscle cells in collagen lattice
Vascular endothelial and smooth muscle cell galvanotactic response and differential migratory behavior
Expression of smooth muscle and non-muscle myosin heavy chain isoforms in cultured vascular smooth muscle cells
Journal Article
·
Fri Dec 09 23:00:00 EST 2005
· Experimental Cell Research
·
OSTI ID:20775322
Vascular endothelial and smooth muscle cell galvanotactic response and differential migratory behavior
Journal Article
·
Sun Feb 14 23:00:00 EST 2021
· Experimental Cell Research
·
OSTI ID:23195384
Expression of smooth muscle and non-muscle myosin heavy chain isoforms in cultured vascular smooth muscle cells
Journal Article
·
Tue Mar 04 23:00:00 EST 1986
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
·
OSTI ID:6763784
Related Subjects
550301* -- Cytology-- Tracer Techniques
551001 -- Physiological Systems-- Tracer Techniques
560121 -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BODY
CELL CULTURES
ENDOTHELIUM
EXTERNAL IRRADIATION
GLOBULINS
HEALING
IMMUNE SERUMS
IN VITRO
INJURIES
IRRADIATION
MIGRATION
MYOSIN
ORGANIC COMPOUNDS
PHYSIOLOGY
PROTEINS
RADIATION EFFECTS
RECOVERY
TISSUES
TROPOMYOSIN
WOUNDS
551001 -- Physiological Systems-- Tracer Techniques
560121 -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BODY
CELL CULTURES
ENDOTHELIUM
EXTERNAL IRRADIATION
GLOBULINS
HEALING
IMMUNE SERUMS
IN VITRO
INJURIES
IRRADIATION
MIGRATION
MYOSIN
ORGANIC COMPOUNDS
PHYSIOLOGY
PROTEINS
RADIATION EFFECTS
RECOVERY
TISSUES
TROPOMYOSIN
WOUNDS