Characterization of lymphoid cells in the blood of healthy adults: sequential immunological, cytochemical and cytokinetic studies
With a new method, sequential immunological, cytochemical and cytokinetic studies were done on lymphoid cells in the peripheral blood of 12 healthy adults. Every single lymphoid cell could therefore be characterized by the following markers: surface immunoglobulins (sIg); rosetting with sheep red blood cells (E); unspecific acid alpha-naphthyl acetate esterase (ANAE); and 3HdT incorporation. Significantly more E+sIg-ANAE-cells (51% and 22% of all lymphoid cells, respectively). Of all ANAE+ cells 90% were E+, but 64% of all ANAE- cells were also E+. In all individuals a subpopulation of E+sIg+ cells was found. The esterase pattern of these cells was similar to that of E-sIg+ cells. The overall labeling index of the lymphoid cells examined was less than or equal to 0.2%.
- OSTI ID:
- 5639431
- Journal Information:
- Am. J. Hematol.; (United States), Vol. 9:3
- Country of Publication:
- United States
- Language:
- English
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LYMPHOCYTES
CYTOCHEMISTRY
AUTORADIOGRAPHY
BLOOD
CYTOLOGY
ENZYME ACTIVITY
ESTERASES
IMMUNOGLOBULINS
LABELLING
MAN
TRITIUM COMPOUNDS
UPTAKE
ANIMAL CELLS
ANIMALS
BIOCHEMISTRY
BIOLOGICAL MATERIALS
BIOLOGY
BLOOD CELLS
BODY FLUIDS
CHEMISTRY
CONNECTIVE TISSUE CELLS
ENZYMES
GLOBULINS
HYDROLASES
LABELLED COMPOUNDS
LEUKOCYTES
MAMMALS
MATERIALS
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
SOMATIC CELLS
VERTEBRATES
550301* - Cytology- Tracer Techniques
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