ATP citrate lyase from germinating castor bean endosperm: localization and some properties

Journal Article · Sun Apr 01 04:00:00 EST 1979 · Plant Physiol.; (United States)

DOI:https://doi.org/10.1104/pp.63.4.687· OSTI ID:5639327

Fritsch, H; Beevers, H

ATP citrate lyase (EC 4.1.3.8) has been found in crude extracts from endosperm tissue of germinating castor bean and shows its maximum activity in 4- to 5-day-old seedlings. A strict requirement for coenzyme A and adenosine 5'-triphosphate was demonstrated. The pH optimum for the reaction is around 7.5. The unstable enzyme can be stabilized by freezing and addition of citrate and glycerol. A potent inhibitor is (-)-hydroxycitrate. The molecular weight is about 400,000. The adenosine 5'-triphosphate citrate lyase is localized in the plastids, where it possibly plays a role in providing acetyl coenzyme A for lipid biosynthesis.

• Research Organization: Univ. of California, Santa Cruz
• DOE Contract Number: EY-76-S-03-0034
• OSTI ID: 5639327
• Journal Information: Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 63:4; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ACETYL RADICALS
ACYL RADICALS
ALCOHOLS
ATP
BEANS
BIOSYNTHESIS
CARBOXYLIC ACID SALTS
CASTOR
CITRATES
COENZYMES
ENDOSPERM
ENZYME INHIBITORS
ENZYMES
FOOD
FREEZING
GLYCEROL
HYDROXY COMPOUNDS
HYDROXYL RADICALS
LIPIDS
LYASES
MEDICINAL PLANTS
NUCLEOTIDES
ORGANIC COMPOUNDS
PH VALUE
PLANT TISSUES
PLANTS
RADICALS
SEEDLINGS
SYNTHESIS
VEGETABLES