Intraglomerular thrombotic tendency and glomerular ADPase. Unilateral impairment of ADPase elicits a proaggregatory microenvironment in experimental glomerulonephritis

Journal Article · Mon Apr 01 04:00:00 EST 1991 · Laboratory Investigation (A Journal of Experimental Methods and Pathology); (USA)

OSTI ID:5639300

Poelstra, K; Baller, J F; Hardonk, M J; Bakker, W W ^[1]

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1. University of Groningen (Netherlands)

It has been proposed, predominantly from ex vivo studies, that glomerular ADPase may function as an antithrombotic principle within the rat kidney. Therefore, intraglomerular platelet aggregation was studied in vivo in rats after impairment of glomerular ADPase activity using local X-irradiation (20 Gy). Biochemical assays in suspensions of glomeruli obtained from rats 24 hours after local X-irradiation (group I) demonstrated a significant reduction in ADPase activity as compared to sham treated rats (group II; p less than 0.01). Cytochemical observations at the ultrastructural level showed that this reduction in glomerular enzyme activity represents in particular ADPase activity detectable in the basement membrane. Following X-irradiation, intraglomerular platelet aggregation was quantitatively studied in two groups of rats. Both groups received X-irradiation of the left kidney (20 Gy). Twenty-four hours after X-irradiation, animals received an intravenous injection of either 0.5 ml of saline (group III; N = 6) or 0.5 ml of heterologous nephrotoxic serum (NTS; group IV; N = 6). Subsequently, 24 hours after this injection, platelet aggregation in left kidneys was compared with aggregation in contralateral non-X-irradiated kidneys. The results showed that while X-irradiation per se did not induce intraglomerular platelet aggregation as compared with the contralateral kidney (0.20 +/- 0.08% versus 0.17 +/- 0.06% platelet aggregation/glomerulus), a significant increase in platelet aggregation could be demonstrated in X-irradiated kidneys in the early phase of nephrotoxic serum nephritis as compared with the contralateral nephritic kidney. A potential effect of altered influx of inflammatory cells after X-irradiation could be excluded since no difference in H2O2 producing cells was observed between left and right kidneys.

• OSTI ID: 5639300
• Journal Information: Laboratory Investigation (A Journal of Experimental Methods and Pathology); (USA), Journal Name: Laboratory Investigation (A Journal of Experimental Methods and Pathology); (USA) Vol. 64:4; ISSN 0023-6837; ISSN LAINA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACID ANHYDRASES
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BLOOD COAGULATION
BLOOD PLATELETS
BODY
BODY FLUIDS
DISEASES
DRUGS
ELECTROMAGNETIC RADIATION
ENZYME ACTIVITY
ENZYMES
FIBRINOLYTIC AGENTS
HEMATOLOGIC AGENTS
HYDROGEN COMPOUNDS
HYDROGEN PEROXIDE
HYDROLASES
IONIZING RADIATIONS
KIDNEYS
MAMMALS
MATERIALS
NEPHRITIS
ORGANS
OXYGEN COMPOUNDS
PEROXIDES
PHOSPHOHYDROLASES
RADIATION EFFECTS
RADIATIONS
RATS
RODENTS
UROGENITAL SYSTEM DISEASES
VERTEBRATES
X RADIATION