Effect of nitrogen dioxide on synthesis of inflammatory cytokines expressed by human bronchial epithelial cells in vitro

Journal Article · Wed Sep 01 00:00:00 EDT 1993 · American Journal of Respiratory Cell and Molecular Biology; (United States)

DOI:https://doi.org/10.1165/ajrcmb/9.3.271· OSTI ID:5634436

Devalia, J L; Campbell, A M; Sapsford, R J; Rusznak, C; Quint, D; Godard, P; Bousquet, J; Davies, R J ^[1]

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1. St. Bartholomew's Hospital, London (United Kingdom)

Although studies of nitrogen dioxide (NO2) inhalation, in both animals and humans, have demonstrated that this agent can cause epithelial cell damage and inflammation of the airway epithelium, the mechanisms underlying these effects are not well understood. We have cultured human bronchial epithelial cells, as explant cultures from surgical tissue, and studied these firstly from their ability to constitutively synthesize specific proinflammatory cytokines and then investigated the effect of exposure to NO2 on the generation of these cytokines. Constitutive synthesis of cytokines was evaluated by analysis of both the expression of the mRNA for interleukin (IL)-1 beta, IL-4, IL-8, granulocyte/macrophage colony-stimulating factor (GM-CSF), tumor necrosis factor-alpha (TNF-alpha), and interferon-gamma (IFN-gamma), by the polymerase chain reaction (PCR), and by immunocytochemical staining for the presence of cell-associated IL-1 beta, IL-8, GM-CSF, TNF-alpha, and IFN-gamma, using specific monoclonal and polyclonal antibodies directed towards these cytokines. Release of IL-4, IL-8, GM-CSF, TNF-alpha, and IFN-gamma following exposure to 5% CO2 in air or 400 ppb and 800 ppb NO2 for 6 h was investigated by enzyme-linked immunosorbent assay. PCR demonstrated that the human bronchial epithelial cells expressed the mRNA for IL-1 beta, IL-8, GM-CSF, and TNF-alpha but not for IL-4 and IFN-gamma. Immunocytochemical staining confirmed the presence of endogenous IL-1 beta, IL-8, GM-CSF, and TNF-alpha.

• OSTI ID: 5634436
• Journal Information: American Journal of Respiratory Cell and Molecular Biology; (United States), Journal Name: American Journal of Respiratory Cell and Molecular Biology; (United States) Vol. 9:3; ISSN 1044-1549; ISSN AJRBEL
• Country of Publication: United States
• Language: English

Related Subjects

550300 -- Cytology
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADULTS
AGE GROUPS
AGED ADULTS
ANIMAL TISSUES
ANTIBODIES
BIOASSAY
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BODY
BRONCHI
CHALCOGENIDES
CHEMISTRY
CYTOCHEMISTRY
DNA
ELDERLY PEOPLE
ENZYME IMMUNOASSAY
EPITHELIUM
GROWTH FACTORS
HUMAN POPULATIONS
IMMUNOASSAY
INFLAMMATION
INHALATION
INTAKE
INTERFERON
LYMPHOKINES
MINORITY GROUPS
MITOGENS
NECROSIS
NITROGEN COMPOUNDS
NITROGEN DIOXIDE
NITROGEN OXIDES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PATHOLOGICAL CHANGES
POPULATIONS
PROTEINS
RESPIRATORY SYSTEM
SYMPTOMS
TISSUES