Labelling index of human and mouse tumors assessed by bromodeoxyuridine staining in vitro and in vivo and flow cytometry
Journal Article
·
· Cytometry (Baltimore); (United States)
OSTI ID:5634245
Bromodeoxyuridine (BrdUrd) incorporation and flow cytometry were used to measure human tumor kinetic parameters in vitro and in vivo. The technique was validated by comparison of labelling index estimates of mouse tumors in vivo and in vitro using BrdUrd and flow cytometry with tritiated thymidine (/sup 3/HdThd) autoradiography. Similar labelling indices were obtained with both in vivo and in vitro incorporation into DNA of the two different precursors. Measurements of human tumor labelling indices were similar following in vitro incubation with either BrdUrd or /sup 3/HdThd. The use of BrdUrd allowed the visualization of a population of S-phase cells that did not appear to incorporate BrdUrd or /sup 3/HdThd. The human tumor labelling indices obtained with BrdUrd incorporation were similar to previously reported values using autoradiography studies. Preliminary studies demonstrated that significant human tumor labelling could be achieved with an intravenous injection of 500 mg BrdUrd.
- OSTI ID:
- 5634245
- Journal Information:
- Cytometry (Baltimore); (United States), Journal Name: Cytometry (Baltimore); (United States) Vol. 6; ISSN CYTOD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANTIMETABOLITES
AUTORADIOGRAPHY
AZINES
BROMOURACILS
BUDR
CELL CYCLE
CELL FLOW SYSTEMS
DISEASES
DRUGS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IN VITRO
IN VIVO
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LABELLING
MAMMALS
MICE
NEOPLASMS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RIBOSIDES
RODENTS
STAINS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
URACILS
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANTIMETABOLITES
AUTORADIOGRAPHY
AZINES
BROMOURACILS
BUDR
CELL CYCLE
CELL FLOW SYSTEMS
DISEASES
DRUGS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IN VITRO
IN VIVO
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LABELLING
MAMMALS
MICE
NEOPLASMS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RIBOSIDES
RODENTS
STAINS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
URACILS
VERTEBRATES