Fluorescence detection and identification of nitro derivatives of polynuclear aromatic hydrocarbons by on-column catalytic reduction to aromatic amines
Nitro derivatives of polynuclear aromatic hydrocarbons (nitro-PAH) at trace-level concentrations were efficiently reduced to primary aromatic amines on a catalyst column packed with 5-..mu..m alumina that was coated with platinum and rhodium (Pt/Rh). The reduction was accomplished in a reversed-phase HPLC system using methanol/water for the mobile phase. Reduction is classically catalytic; i.e., the Pt/Rh catalyst is not consumed. Fluorescence measurements permitted detection of some nitro-PAH as aromatic amines at low picogram levels. Excitation and emission wavelengths for fluorescence detection of 19 aromatic amines are given along with their fluorescence spectra. Some spectra exhibit unique features that are useful for confirming identification. An analytical system based on multidimensional HPLC principles was developed for routine analysis of specific nitro-PAH in complex organic matrices such as extracts of diesel exhaust particulates with minimal sample preparation.
- Research Organization:
- Environmental Protection Agency, Research Triangle Park, NC
- OSTI ID:
- 5630669
- Journal Information:
- Anal. Chem.; (United States), Journal Name: Anal. Chem.; (United States) Vol. 58:8; ISSN ANCHA
- Country of Publication:
- United States
- Language:
- English
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54 ENVIRONMENTAL SCIENCES
ALCOHOLS
AMINES
AROMATICS
CATALYSIS
CATALYTIC EFFECTS
CHEMICAL ANALYSIS
CHEMICAL PREPARATION
CHEMICAL REACTIONS
CHROMATOGRAPHY
DATA
DIESEL FUELS
ELEMENTS
EMISSION SPECTROSCOPY
EXHAUST GASES
EXPERIMENTAL DATA
FLUIDS
FLUORESCENCE SPECTROSCOPY
GASEOUS WASTES
GASES
HYDROCARBONS
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
INFORMATION
LIQUID COLUMN CHROMATOGRAPHY
METALS
METHANOL
NITRO COMPOUNDS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN COMPOUNDS
PARTICLES
PARTICULATES
PETROLEUM PRODUCTS
PLATINUM
PLATINUM METALS
POLYCYCLIC AROMATIC HYDROCARBONS
POLYCYCLIC NITRO COMPOUNDS
QUALITATIVE CHEMICAL ANALYSIS
REDUCTION
RHODIUM
SEPARATION PROCESSES
SPECTROSCOPY
SYNTHESIS
TRACE AMOUNTS
TRANSITION ELEMENTS
WASTES
WATER