Biosynthesis of the phytoalexin pisatin. [Pisum sativum]

Journal Article · Thu Nov 01 00:00:00 EST 1990 · Plant Physiology; (USA)

DOI:https://doi.org/10.1104/pp.94.3.1444· OSTI ID:5628659

Preisig, C L; Bell, J N; Matthews, D E; VanEtten, H D ^[1]; Sun, Yuejin; Hrazdina, G ^[2]

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1. Cornell Univ., Ithaca, NY (USA)
2. Cornell Univ., Geneva, NY (USA)

NADPH-dependent reduction of 2{prime},7-dihydroxy-4{prime},5{prime}-methylenedioxyisoflavone to the isoflavanone sophorol, a proposed intermediate step in pisatin biosynthesis, was detected in extracts of Pisum sativum. This isoflavone reductase activity was inducible by treatment of pea seedlings with CuCl{sub 2}. The timing of induction coincided with that of the 6a-hydroxymaackiain 3-O-methyltransferase, which catalyzes the terminal biosynthetic step. Neither enzyme was light inducible. Further NADPH-dependent metabolism of sophorol by extracts of CuCl{sub 2}-treated seedlings was also observed; three products were radiolabeled when ({sup 3}H)sophorol was the substrate, one of which is tentatively identified as maackiain.

• OSTI ID: 5628659
• Journal Information: Plant Physiology; (USA), Vol. 94:3; ISSN 0032-0889
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PISUM
PHYSIOLOGY
PLANT GROWTH REGULATORS
BIOSYNTHESIS
COPPER CHLORIDES
ENZYME ACTIVITY
METHYL TRANSFERASES
NADP
OXIDOREDUCTASES
SEEDLINGS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
CHLORIDES
CHLORINE COMPOUNDS
COENZYMES
COPPER COMPOUNDS
COPPER HALIDES
ENZYMES
HALIDES
HALOGEN COMPOUNDS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
LEGUMINOSAE
MAGNOLIOPHYTA
MAGNOLIOPSIDA
NUCLEOTIDES
ORGANIC COMPOUNDS
PLANTS
SYNTHESIS
TRANSFERASES
TRANSITION ELEMENT COMPOUNDS
550201* - Biochemistry- Tracer Techniques