Isolation of a photosystem II reaction center consisting of D-1 and D-2 polypeptides and cytochrome b-559
Journal Article
·
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
A photosystem II reaction center complex consisting of D-1 and D-2 polypeptides and cytochrome b-559 was isolated from spinach grana thylakoids, treated with 4% (wt/vol) Triton X-100, by ion-exchange chromatography using DEAE-Toyopearl 650S. The isolated complex appears to contain five chlorophyll a, two pheophytin a, one ..beta..-carotene, and one or two cytochrome b-559 heme(s) (molar ratio) and exhibits a reversible absorbance change attributable to the photochemical accumulation of reduced pheophytin typical for the intermediary electron acceptor of photosystem II reaction center. These results strongly suggest that the site of primary charge separation in photosystem II is located on the heterodimer composed of D-1 and D-2 subunits.
- Research Organization:
- Okayama Univ., Japan
- OSTI ID:
- 5628501
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:1; ISSN PNASA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
14 SOLAR ENERGY
140505* -- Solar Energy Conversion-- Photochemical
Photobiological
& Thermochemical Conversion-- (1980-)
ABSORPTION SPECTRA
CARBOXYLIC ACIDS
CHLOROPHYLL
CHROMATOGRAPHY
CYTOCHROMES
ELECTRON EXCHANGE
ELECTRON TRANSFER
ELECTROPHORESIS
FOOD
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
ION EXCHANGE CHROMATOGRAPHY
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEPTIDES
PHOTOSYNTHETIC REACTION CENTERS
PHYTOCHROMES
PIGMENTS
PLANTS
POLYPEPTIDES
PORPHYRINS
PROTEINS
PURIFICATION
SEPARATION PROCESSES
SPECTRA
SPINACH
VEGETABLES
140505* -- Solar Energy Conversion-- Photochemical
Photobiological
& Thermochemical Conversion-- (1980-)
ABSORPTION SPECTRA
CARBOXYLIC ACIDS
CHLOROPHYLL
CHROMATOGRAPHY
CYTOCHROMES
ELECTRON EXCHANGE
ELECTRON TRANSFER
ELECTROPHORESIS
FOOD
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
ION EXCHANGE CHROMATOGRAPHY
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEPTIDES
PHOTOSYNTHETIC REACTION CENTERS
PHYTOCHROMES
PIGMENTS
PLANTS
POLYPEPTIDES
PORPHYRINS
PROTEINS
PURIFICATION
SEPARATION PROCESSES
SPECTRA
SPINACH
VEGETABLES