Sequence and expression of ferredoxin mRNA in barley

Conference · Tue May 01 04:00:00 EDT 1990 · Plant Physiology, Supplement; (USA)

OSTI ID:5627942

Zielinski, R; Funder, P M; Ling, V ^[1]

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1. Univ. of Illinois, Urbana (USA)

We have isolated and structurally characterized a full-length cDNA clone encoding ferredoxin from a {lambda}gt10 cDNA library prepared from barley leaf mRNA. The ferredoxin clone (pBFD-1) was fused head-to-head with a partial-length cDNA clone encoding calmodulin, and was fortuitously isolated by screening the library with a calmodulin-specific oligonucleotide probe. The mRNA sequence from which pBFD-1 was derived is expressed exclusively in the leaf tissues of 7-d old barley seedlings. Barley pre-ferredoxin has a predicted size of 15.3 kDal, of which 4.6 kDal are accounted for by the transit peptide. The polypeptide encoded by pBFD-1 is identical to wheat ferredoxin, and shares slightly more amino acid sequence similarity with spinach ferredoxin I than with ferredoxin II. Ferredoxin mRNA levels are rapidly increased 10-fold by white light in etiolated barley leaves.

• OSTI ID: 5627942
• Report Number(s): CONF-9007196--
• Conference Information: Journal Name: Plant Physiology, Supplement; (USA) Journal Volume: 93:1
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
BARLEY
CALMODULIN
CEREALS
CLONING
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
ELECTROMAGNETIC RADIATION
FERREDOXIN
GRAMINEAE
HYBRIDIZATION
LILIOPSIDA
MAGNOLIOPHYTA
MESSENGER-RNA
METALLOPROTEINS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PEPTIDES
PLANTS
POLYPEPTIDES
PROTEINS
RADIATIONS
RNA
SEEDLINGS
STRUCTURAL CHEMICAL ANALYSIS
VISIBLE RADIATION