The gene required for inorganic carbon transport of Synechocystis PCC6803

Conference · Tue May 01 04:00:00 EDT 1990 · Plant Physiology, Supplement; (USA)

OSTI ID:5627838

Ogawa, Teruo ^[1]

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1. RIKEN, Saitama (Japan)

Cyanobacteria possess a CO{sub 2}-concentrating mechanism which involves active transport of inorganic carbon (C{sub i}). Two mutants (RKa and RKb) of {und Synechocystis} PCC6803 defective in C{sub i} transport have been isolated. These mutants required high CO{sub 2} for growth. A clone which complements RKb contained a 5.2 kbp DNA insert. By subcloning, we have constructed a complementing clone with a 1.2 kbp DNA insert. Sequencing of the insert DNA revealed an open reading frame (ORF) which codes a hydrophobic protein consists of 80 amino acids. Insertion of kanamycin resistance gene cartridge into the ORF in the wild-type cells of {und Synechocystis} led to the loss of C{sub i}-transport activity. Sequenching of DNA in the same region of the genome in RKb indicated that the mutation is the change of AAA (Lys) to TAA (stop codon). The clone did not complement RKa. Isolation of a clone which complements RKa is in progress.

• OSTI ID: 5627838
• Report Number(s): CONF-9007196--
• Conference Information: Journal Name: Plant Physiology, Supplement; (USA) Journal Volume: 93:1
• Country of Publication: United States
• Language: English

Related Subjects

550400 -- Genetics
550500* -- Metabolism
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CARBOXYLIC ACIDS
CHALCOGENIDES
CYANOBACTERIA
DNA SEQUENCING
DRUGS
INORGANIC COMPOUNDS
METABOLISM
MICROORGANISMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PROTEINS
STRUCTURAL CHEMICAL ANALYSIS