Effect of NO/sub 2/ inhalation and vitamin C deficiency on protein and lipid accumulation in the lung

Journal Article · Tue Dec 01 04:00:00 EST 1981 · Environ. Res.; (United States)

OSTI ID:5627736

Selgrade, M K; Mole, M L; Miller, F J; Hatch, G E; Gardner, D E; Hu, P C

Vitamin C-deficient and normal guinea pigs were exposed to various concentrations of NO/sub 2/ or air, and lavage fluid was obtained and analyzed for protein and lipid content. Exposure of normal animals to 752, 1880, 5640, or 9400 ..mu..g NO/sub 2//m/sup 3/ (0.4, 1.0, 3.0, or 5.0 ppm) for 72 hr did not alter the protein or lipid content of lung lavage fluid. However, exposure of vitamin C-deficient animals to the same concentrations of NO/sub 2/ caused marked increases in lavage proteins and lipids at all but the 752 ..mu..g/m/sup 3/ (0.4 ppm) level. At 9400 ..mu..g NO/sub 2//m/sup 3/ (5.0 ppm), 50% of the exposed C-deficient animals died, and pathologic study of the lungs showed proteinaceous edema fluid in the alveoli. Lungs from air-exposed animals and normal animals exposed to NO/sub 2/ appeared healthy. No effects were seen at 752 ..mu..g NO/sub 2/ (0.4 ppm) in either normal or deficient animals even when the time of exposure was extended to 1 week. At 9400 ..mu..g NO/sub 2//m/sup 3/ (5 ppm) effects could be seen in vitamin C-deficient animals even when the exposure period was shortened to 3 hr. Assessment of protein and lipid content of lavage fluid provided a sensitive method for determining subtle changes in the lung following NO/sub 2/ exposure.

• Research Organization: Environmental Protection Agency, Research Triangle Park, NC
• OSTI ID: 5627736
• Journal Information: Environ. Res.; (United States), Journal Name: Environ. Res.; (United States) Vol. 26:2; ISSN ENVRA
• Country of Publication: United States
• Language: English

Related Subjects

560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
ASCORBIC ACID
BIOLOGICAL EFFECTS
BODY
CHALCOGENIDES
GUINEA PIGS
INHALATION
INTAKE
LIPIDS
LUNGS
MAMMALS
NITROGEN COMPOUNDS
NITROGEN DIOXIDE
NITROGEN OXIDES
NUTRITIONAL DEFICIENCY
ORGANIC COMPOUNDS
ORGANS
OXIDES
OXYGEN COMPOUNDS
PROTEINS
RESPIRATORY SYSTEM
RODENTS
VERTEBRATES
VITAMINS