(Genetic engineering of corn and other higher plants): Final technical report
Two uses for restriction fragment length polymorphisms associated with multigene families in the corn genome were demonstrated. RFLPs detected by a DNA probe (cZm7) homologous to a multigene family of unidentified function were used as genetic markers for the identification of inbred lines. A rapid small-scale procedure was presented for isolating DNA from individual corn seedlings suitable for digestion by restriction endonucleases. When hybridized to inbred line DNAs digested with a single restriction enzyme, the cZm7 probe detects unique patterns in 47 out of 49 (96%) inbred lines widely used in corn breedings. Linkage analysis of RFLPs associated with a second multigene family was used to determine the genomic organization of the gene family. Methods were outlined for identifying alleles of loci belonging to multigene families that were polymorphic by analyzing the DNAs of a relatively small number of F/sub 2/ segregants. These approaches were generally useful for the genetic analysis of multigene families in diploid organisms for which a DNA probe and individuals exhibiting RFLPs detected by the probe were available.
- Research Organization:
- Stanford Linear Accelerator Center, Menlo Park, CA (USA)
- DOE Contract Number:
- FG03-84ER13265
- OSTI ID:
- 5625779
- Report Number(s):
- DOE/ER/13265-T1; ON: DE88004497
- Resource Relation:
- Other Information: Portions of this document are illegible in microfiche products
- Country of Publication:
- United States
- Language:
- English
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60 APPLIED LIFE SCIENCES
59 BASIC BIOLOGICAL SCIENCES
MAIZE
GENETIC MAPPING
GENETIC VARIABILITY
PROGRESS REPORT
RFLPS
BIOLOGICAL VARIABILITY
CEREALS
DOCUMENT TYPES
GRASS
MAPPING
PLANTS
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