Nitroaromatics are substrates for the TOL plasmid upper-pathway enzymes

Journal Article · Wed Jan 01 04:00:00 EST 1992 · Applied and Environmental Microbiology; (United States)

OSTI ID:5624262

Delgado, A; Abril, M A; Ramos, J L ^[1]; Wubbolts, M G ^[2]

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1. Consejo Superior de Investigaciones Cientificas, Granada (Spain)
2. Univ. of Groningen (Netherlands)

Expression of the xylMA genes encoding for toluene monoxygenase from the lactose promoter in a broad-host-range plasmid allows the oxidation of toluene and m- and p-nitrotoluene to their corresponding benzyl alcohols and benzaldehydes in Pseudomonas putida and Escherichia coli. Benzyl alcohols accumulate until reaching a concentration of about 80 {mu}M, while benzaldehydes accumulate steadily with time for at least 24 h. TOL-encoded benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase recognize m- and p-nitro-substituted compounds as substrates. In contrast, the XylR protein, which regulates the TOL plasmid-encoded upper-pathway operon, does not recognize nitro-substituted toluenes as effectors.

• OSTI ID: 5624262
• Journal Information: Applied and Environmental Microbiology; (United States), Journal Name: Applied and Environmental Microbiology; (United States) Vol. 58:1; ISSN 0099-2240; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
540210* -- Environment
Terrestrial-- Basic Studies-- (1990-)
ALCOHOLS
ALKYLATED AROMATICS
AROMATICS
BACTERIA
BIODEGRADATION
BIOLOGICAL PATHWAYS
CELL CONSTITUENTS
CHEMICAL REACTIONS
DECOMPOSITION
ENZYMES
ESCHERICHIA COLI
GENE OPERONS
GENES
HYDROCARBONS
HYDROXY COMPOUNDS
METABOLISM
MICROORGANISMS
NITRO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDOREDUCTASES
PLASMIDS
PROTEINS
PSEUDOMONAS
SOILS
SUBSTRATES
TOLUENE